Carboxyl-rich plasma polymer surfaces in surface plasmon resonance immunosensing

Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing th...

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 2018-01, Vol.57 (1S), p.1
Main Authors: Makhneva, Ekaterina, Obrusník, Adam, Farka, Zden k, Skládal, Petr, Vandenbossche, Marianne, Hegemann, Dirk, Zají ková, Lenka
Format: Article
Language:English
Subjects:
Biomolecules
Flow stability
Gold
Immunoassay
Immunosensors
Monoclonal antibodies
Serum albumin
Surface plasmon resonance
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Summary:Stable carboxyl-rich plasma polymers (PPs) were deposited onto the gold surface of surface plasmon resonance (SPR) chips under conditions that were chosen based on lumped kinetic model results. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity, allowing the formation of covalent linkages between biomolecules and a surface. Accordingly, the monoclonal antibody, specific to human serum albumin (HSA), was immobilized and the performance of SPR immunosensors was evaluated by the immunoassay flow test. The developed sensors performed high level of stability and provided selective and high response to the HSA antigen solutions. The achieved results confirmed that the presented methodologies for the grafting of biomolecules on the gold surfaces have great potential for biosensing applications.
ISSN:0021-4922
1347-4065
DOI:10.7567/JJAP.57.01AG06