Reconstituting donor T cells increase their biomass following hematopoietic stem cell transplantation

In this study, we used a rapid, highly-sensitive, single-cell biomass measurement method, Live Cell Interferometry (LCI), to measure biomass in populations of CD3 + T cells isolated from hematopoietic stem cell transplant (SCT) patients at various times pre- and post-transplant (days 0-100). CD3 + T...

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Bibliographic Details
Published in:Analyst (London) 2018-05, Vol.143 (11), p.2479-2485
Main Authors: Leslie, Kevin A, Rasheed, Mahmood, Sabo, Roy T, Roberts, Catherine C, Toor, Amir A, Reed, Jason
Format: Article
Language:English
Subjects:
Biomass
Cell Size
Graft vs Host Disease
Hematopoietic Stem Cell Transplantation
Humans
Immunosuppression
Interferometry
Lymphocytes
Mass spectra
Medical research
Stem cells
T-Lymphocytes - cytology
Transplantation
Transplantation, Homologous
Transplants & implants
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Summary:In this study, we used a rapid, highly-sensitive, single-cell biomass measurement method, Live Cell Interferometry (LCI), to measure biomass in populations of CD3 + T cells isolated from hematopoietic stem cell transplant (SCT) patients at various times pre- and post-transplant (days 0-100). CD3 + T cell 'mass spectra' were obtained from five autologous and 20 allogenic transplant recipients. We found a pronounced rise in median T cell biomass (+25%; p
ISSN:0003-2654
1364-5528
DOI:10.1039/c8an00148k