Analyses of the results of different test systems in the 2005 global proficiency testing schemes for infectious bursal disease virus and Newcastle disease virus antibody detection in chicken serum

• Published in: Avian pathology 2007-04, Vol.36 (2), p.177-183
• Main Authors: Wit, J.J. de, Sande, H.W.A. van de, Counotte, G.H.M, Wellenberg, G.J
• Format: Article
• Language: English
• Subjects: accuracy; agar gel precipitation tests; Animal diseases; Animals; antibodies; Antibodies, Viral - blood; Birnaviridae Infections - epidemiology; Birnaviridae Infections - immunology; Birnaviridae Infections - veterinary; Body fluids; Chickens - blood; Chickens - immunology; Chickens - virology; diagnostic techniques; disease detection; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; geographical variation; Global Health; hemagglutination inhibition test; Hemagglutination Tests - methods; Hemagglutination Tests - veterinary; Immunoassay; Immunoglobulins; Infectious bursal disease virus; Infectious bursal disease virus - immunology; Infectious diseases; laboratories; Laboratories - standards; laboratory techniques; neutralization tests; Newcastle Disease - epidemiology; Newcastle Disease - immunology; Newcastle disease virus; Newcastle disease virus - immunology; Pathology; Poultry; Poultry Diseases - blood; Poultry Diseases - epidemiology; Poultry Diseases - immunology; Poultry Diseases - virology; reference standards; serodiagnosis; seroprevalence; vertebrate viruses; Veterinary services; viral antigens; Viruses
• ISSN: 0307-9457; 1465-3338
• DOI: 10.1080/03079450601105676

The results of global proficiency testing schemes (PTS) for serological tests to detect antibodies against infectious bursal disease virus (IBDV) and Newcastle disease virus (NDV) in chicken serum, in which 125 and 120 laboratories, respectively, participated from Africa, Asia, Europe, Central and South America, were used to analyse the performances of different antibody test systems such as virus neutralization tests, haemagglutination inhibition tests, enzyme-linked immunosorbent assays and agar gel precipitation tests. All laboratories were asked to carry out their routine diagnostic tests for the detection of IBDV and NDV antibodies as usual. This global ring trial provided a large amount of data on variation within and between laboratories and test systems used worldwide. The data showed that the variation between the quantitative test results of different laboratories (Rbetween) using the IBDV virus neutralization tests and the NDV haemagglutination inhibition test was higher (about double) compared with the variation within commercial enzyme-linked immunosorbent assay systems. Although both tests are often referred to and used as the "gold standard" in experimental and scientific studies, official procedures and for the validation of tests, this study shows that there is an urgent need for a global implementation of recommended test procedures and/or the inclusion of international reference sera in these studies.