Inulin as prebiotic for Lactobacillus salivarius and Enterococcus faecium with probiotic potential in ruminants

• Published in: Journal of animal science 2016-10, Vol.94, p.794-795
• Main Authors: Hernández-Sánchez, D, Gómez–Hernández, J L, Crosby–Galván, M M, Hernández-Anguiano, A M, Ramirez-Bribiesca, J E, Aranda–Ibañez, E, Gonzalez-Muñoz, S S, Pinto-Ruiz, R
• Format: Article
• Language: English
• Subjects: Acid production; Acid resistance; Ammonium; Antagonism; Bacteria; Bile salts; Calves; Cattle; Data processing; Diarrhea; Digestive system; E coli; Enterococcus faecium; Experimental design; Fermented food; Flora; Gastrointestinal tract; Glucose; Hydrochloric acid; Inulin; Lactic acid; Lactic acid bacteria; Lactobacilli; Lactobacillus; Milk production; Probiotics; Salmonella; Salts; Strains (organisms); Suckling behavior
• ISSN: 0021-8812; 1525-3163

Milk production in Mexico is deficient and there are diarrhea problems in nursing calves. Lactic acid bacteria (LAB) are found in the digestive tract and show an antagonistic effect against enteric pathogens. Addition of prebiotics such as inulin to diets of calves might control gastrointestinal flora. Therefore, the aim of this research was to evaluate the influence of inulin on in vitro growth performance of Lactobacillus casei (Lc), Lactobacillus salivarius (Ls), and Enterococcus faecium (Ef). In vitro incubations were performed at 37°C, replacing the MRS glucose for inulin. The experimental design was complete randomized and treatments (T) were: T1 = MRS-glucose + Ls; T2 = MRS-glucose + Ef; T3 = MRS-glucose + Lc; T4 = MRS-inulin + Ls; T5 = MRS-inulin + Ef; T6 = MRS-inulin + Lc; T7 = MRS-inulin + Ls + Ef; T8 = MRS-inulin + Ls + Lc; T9 = MRS-inulin + Ef + Lc; and T10 = MRS-inulin + Ls + Ef + Lc. Variables were growth curve, pH, lactic acid production, ammonium, strains resistance to hydrochloric acid and bile salts, and antagonism against Escherichia coli and Salmonella typhimurium. Data were statistically analyzed with PROC GLM of SAS, and Tukey test (P < 0.05) was used to compare treatments means. Analysis of results showed a positive effect of inulin on the growth of strains, higher absorbance readings in MRS-inulin as compared to MRS-glucose (2.35d, 2.28d, 2.30d, 2.83abc, 2.67c, 2.64c, 2.75abc, 2.93a, 2.72bc, and 2.88ab, from T1 to T10, respectively; P < 0.05) and higher bacterial count at the end of the growth curve (10.98d, 10.76d, 11.29d, 13.11c, 13.63b, 13.77a, 12.93c, 12.74c, 12.43c, and 12.92c Log 10 UFC mL-1 from T1 to T10, respectively; P < 0.05), whereas no changes were found for the other variables. We conclude that LAB Ls and Ef fermented inulin with a positive effect on strains growth, without affecting their probiotic potential.