A new stability indicating RP-HPLC method for the simultaneous estimation of Diloxanide and Ornidazole in bulk and Pharmaceutical Dosage forms

Method Validation: The method is validated as per ICH guidelines like system suitability parameters, linearity, precision, accuracy, limit of detection, limit of quantization, robustness, ruggedness, analysis of marketed samples and forced degradation studies. Development of RP-HPLC method for simul...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2017-12, Vol.10 (12), p.4247-4254
Main Authors: Devi, M. Seetha, Babu, G. Raveendra, Mulukuri, NVL Sirisha
Format: Article
Language:English
Subjects:
Asymmetry
Chromatography
Drug dosages
International conferences
Methods
Pharmaceuticals
Software
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Summary:Method Validation: The method is validated as per ICH guidelines like system suitability parameters, linearity, precision, accuracy, limit of detection, limit of quantization, robustness, ruggedness, analysis of marketed samples and forced degradation studies. Development of RP-HPLC method for simultaneous estimation of Diloxanide and Ornidazole: The separation was done on Discovery column (250 mm x 4.6 mm x 5 gm) and the mobile phase orthophospharic acid buffer and acetonitrile (50:50, v/v) was found to be more satisfactory because of good resolution of drugs with markedly symmetrical sharp peak. [...]the method is specific. The concentrations of Diloxanide and Ornidazole, its respective peak areas were subjected to regression analysis by least squares method, a good linear relationship (r2 = 0.9992) and (r2 = 0.9993) was observed between the concentration of Diloxanide and Ornidazole and the respective peak areas in the range of 81.25-487.5 pg/ml for Diloxanide and 62.5-375 pg/ml for Ornidazole.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.5958/0974-360X.2017.00778.8