Molecular characterization of a stress-induced NAC gene, GhSNAC3, from Gossypium hirsutum

NAC genes, specific to plants, play important roles in plant development as well as in response to biotic and abiotic stresses. Here, a novel gene encoding a NAC domain, named as GhSNAC3 , was isolated from upland cotton ( Gossypium hirsutum L.). Sequence analyses showed that GhSNAC3 encodes a prote...

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Published in:Journal of genetics 2018-06, Vol.97 (2), p.539-548
Main Authors: Liu, Zhan-Ji, Li, Fei, Wang, Li-Guo, Liu, Ren-Zhong, Ma, Jun-Jun, Fu, Ming-Chuan
Format: Article
Language:English
Subjects:
Abscisic acid
Abscisic Acid - pharmacology
Amino Acid Sequence
Animal Genetics and Genomics
Base Sequence
Biomedical and Life Sciences
Cotton
Drought
Droughts
Evolutionary Biology
Gene Expression Regulation, Plant - drug effects
Gene Expression Regulation, Plant - genetics
Genes
Genetic engineering
Genetic transformation
Gossypium - genetics
Gossypium hirsutum
Life Sciences
Localization
Microbial Genetics and Genomics
Nicotiana - genetics
Nuclear Proteins - genetics
Plant Genetics and Genomics
Plant Growth Regulators - pharmacology
Plant Proteins - genetics
Plants, Genetically Modified
Polymerase chain reaction
Research Article
Salinity
Salt Tolerance - genetics
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Tobacco
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Summary:NAC genes, specific to plants, play important roles in plant development as well as in response to biotic and abiotic stresses. Here, a novel gene encoding a NAC domain, named as GhSNAC3 , was isolated from upland cotton ( Gossypium hirsutum L.). Sequence analyses showed that GhSNAC3 encodes a protein of 346 amino acids with an estimated molecular mass of 38.4 kDa and pI of 8.87. Transient localization assays in onion epidermal cells confirmed GhSNAC3 is a nuclear protein. Transactivation studies using a yeast system revealed that GhSNAC3 functions as a transcription activator. Quantitative real-time polymerase chain reaction analysis indicated that GhSNAC3 was induced by high salinity, drought and abscisic acid treatments. We overexpressed GhSNAC3 in tobacco by using Agrobacterium -mediated transformation. Transgenic lines produced longer primary roots and more fresh weight under salt and drought stresses as compared to wild-type plants. Collectively, our results indicated that overexpression of GhSNAC3 in tobacco can enhance drought and salt tolerances.
ISSN:0022-1333
0973-7731
DOI:10.1007/s12041-018-0944-6