Aspergillus ochraceopetaliformis SSP13 modulates quorum sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa PAO1

Pseudomonas aeruginosa is an opportunistic nosocomial pathogen causing the majority of acute and persistent infections in human beings. The ability to form biofilm adds a new dimension to its resistance to conventional therapeutic agents. In the present study, down-regulation of quorum sensing regul...

Full description

Saved in:
Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2018-04, Vol.34 (4), p.410-425
Main Authors: Pattnaik, Subhaswaraj, Ahmed, Tanveer, Ranganathan, Sampath Kumar, Ampasala, Dinakara Rao, Sarma, V. Venkateswara, Busi, Siddharha
Format: Article
Language:English
Subjects:
Aspergillus
Aspergillus ochraceopetaliformis
Attenuation
Bioactive compounds
biofilm
Biofilms
Chemical compounds
Chitinase
Detection
Down-regulation
Elastase
Exopolysaccharides
Hospitals
HPTLC
Human performance
Metabolites
Molecular chains
Molecular docking
Nosocomial infection
Opportunist infection
Pharmacology
Proteins
Pseudomonas aeruginosa
Pyocyanin
Quorum sensing
Receptors
Rhamnolipids
RhlR protein
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pseudomonas aeruginosa is an opportunistic nosocomial pathogen causing the majority of acute and persistent infections in human beings. The ability to form biofilm adds a new dimension to its resistance to conventional therapeutic agents. In the present study, down-regulation of quorum sensing regulated virulence and biofilm development resulting from exposure to Aspergillus ochraceopetaliformis SSP13 extract was investigated. The in vitro results inferred impairment in the production of LasA protease, LasB elastase, chitinase, pyocyanin, exopolysaccharides and rhamnolipids. In addition, motility and biofilm formation by P. aeruginosa PAO1 was significantly altered. The in vitro results were further supported by molecular docking studies of the metabolites obtained from GC-MS analysis depicting the quorum sensing attenuation by targeting the receptor proteins LasR and RhlR. The in vitro and in silico studies suggested new avenues for the development of bioactive metabolites from A. ochraceopetaliformis SSP13 extract as potential anti-infective agents.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2018.1460748