Tip-[alpha] (hp0596 Gene Product) Is a Highly Immunogenic Helicobacter pylori Protein Involved in Colonization of Mouse Gastric Mucosa

A product of the Helicobacter pylori hp0596 gene (Tip-α) is a highly immunogenic homodimeric protein, unique for this bacterium. Cell fractionation experiments indicate that Tip-α is anchored to the inner membrane. In contrast, the three-dimensional model of the protein suggests that Tip-α is solubl...

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Bibliographic Details
Published in:Current microbiology 2008-03, Vol.56 (3), p.279
Main Authors: Godlewska, Renata, Pawlowski, Marcin, Dzwonek, Artur, Mikula, Michal, Ostrowski, Jerzy, Drela, Nadzieja, Jagusztyn-krynicka, Elbieta K
Format: Article
Language:English
Subjects:
Colonization
Fractionation
Genes
Microbiology
Polymerase chain reaction
Proteins
Rodents
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Summary:A product of the Helicobacter pylori hp0596 gene (Tip-α) is a highly immunogenic homodimeric protein, unique for this bacterium. Cell fractionation experiments indicate that Tip-α is anchored to the inner membrane. In contrast, the three-dimensional model of the protein suggests that Tip-α is soluble or, at least, largely exposed to the solvent. hp0596 gene knockout resulted in a significant decrease in the level of H. pylori colonization as measured by real-time PCR assay. In addition, the Tip-α recombinant protein was determined to stimulate macrophage to produce IL-1α and TNF-α. Both results imply that Tip-α is rather loosely connected to the inner membrane and potentially released during infection. [PUBLICATION ABSTRACT]
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-007-9083-7