Protective effect of Gymnemic acid isolated from Gymnema sylvestre leaves coated Chitosan reduced gold nanoparticles in hyperlipedimia and Diabetes Induced vascular tissue damage in Rats

• Published in: Research journal of pharmacy and technology 2018-03, Vol.11 (3), p.1193-1206
• Main Authors: Nirmala, Sankaradoss, Ravichandiran, Velayutham, Vijayalakshmi, A, Nadanasabapathi, P.
• Format: Article
• Language: English
• Subjects: Cardiovascular disease; Diabetes; Gold; Nanoparticles; Nanotechnology
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.5958/0974-360X.2018.00222.6

Objective: To synthesis chitosan reduced gold particle by using gymnemic acid isolated from Gymnema sylvestre leaves and to evaluate the antidiabetic activity against streptozocin and highfat diet treated rats. Methods: In this research paper, the synthesis gold particle by using gymnemic acid isolated from Gymnema sylvestre leaves with chitosan reducer. The synthesized AuNps were determined by UV-vis spectrum, high resolution transmission electron microscopy, X-ray diffraction, and Fourier transmission infrared spectroscopy analysis. The synthesized gymnemic acid coated chitosan reduced gold nanoparticles is subjected to evaluate its antidiabetic activity using high fat diet fed streptozotocin induced type 2 diabetes mellitus rats. Results: UV-vis spectrum showed a peak at 520 nm due to excitation of surface Plasmon vibrations. Fourier transmission infrared spectroscopy showed that nanoparticles were coated with plant secondary metabolites. HR-TEM and AFM images of synthesized AuNPS are spherical in shape and HR-TEM pictures evidently confirms the prepared AuNPS have the uniformed spherical shape with diameter of 14±3.69 nm in size respectively. The result of antidiabetic activity, chitosan reduced gold nano particles shows significant in both invitro and invivo models in high fat diet fed streptozotocin induced type 2 diabetes mellitus