Production, purification and analysis of the isomalto-oligosaccharides from Chinese chestnut (Castanea mollissima Blume) and the prebiotics effects of them on proliferation of Lactobacillus

• Published in: Food and bioproducts processing 2017-11, Vol.106, p.75-81
• Main Authors: Cui, Jie, Li, Yasong, Wang, Qiuling, Li, Jianxia, Ou, Yangjie, Wang, Jianzhong, Wang, Wenquan
• Format: Article
• Language: English
• Subjects: Amylases; Calcium chloride; Carbon sources; Castanea mollissima; Cation exchange; Cation exchanging; Cationic polymerization; Cations; Chemical synthesis; Chestnut; Chestnuts starch; Dextrose; Heat exchange; Ion-exchange chromatography; Isomalto-oligosaccharides; Lactobacillus; Lactobacillus proliferative; Liquefaction; Nuts; Oligosaccharides; Prebiotics; Proliferation; Pullulanase; Raw materials; Resins; Saccharification; Slurries; Starch; Synthesis; Syrup; Syrups; Thermal stability; Transglycosylation; Water purification; α-Amylase; β-Amylase
• ISSN: 0960-3085; 1744-3571
• DOI: 10.1016/j.fbp.2017.08.003

[Display omitted] •The isomalto-oligosaccharides (IMOs) were synthesized by chestnut starch.•The optimal conditions of liquefaction and saccharification were investigated.•This IMOs were purified by ion-exchange chromatography.•The proliferative effects of and chemical compositions of the IMOs were analyzed. The synthesis and stimulating effects of isomalto-oligosaccharides from Chinese chestnut starch (CN-IMOs) on Lactobacillus proliferation were investigated in this paper. The synthesis process of CN-IMOs mainly consisted of liquefaction, saccharification and transglycosylation in sequence. The optimal liquefaction time with heat-stable α-amylase and CaCl2 was 120min, and the dextrose equivalent value of the liquefied slurry was 13.8. Saccharification was performed at 60°C for 2h by using a complex enzyme system comprising fungal α-amylase, β-amylase and pullulanase. The hydrolysate was then subjected to transglycosylation with α-transglucosidase for 20h. The syrup contained 30.53% total CN-IMOs, and then was purified by 732 cation exchange resins (Na+). The recovery rate and total content of it was 91.6% and 61.5%, respectively. Moreover, the proliferative effects of CN-IMOs, purified CN-IMOs (CN-IMOs-P), and commercial isomalto-oligosaccharides (IMOs-50) on Lactobacillus were observed in vitro. CN-IMOs-P was the best effective carbon source for Lactobacillus growth at an optimal dosage of 2.0% in the medium. All the results showed that Chinese chestnut could be a potential raw material for IMOs production, which was a new further processing area of chestnut utilization, and CN-IMOs-P can be explored as a novel prebiotic healthcare product for human gastrointestinal health.