Production of L-dihyroxyphenylalanine in Escherichia coli with the tyrosine phenol-lyase gene cloned from Erwinia herbicola

The gene encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high levels in the presence of isopropyl-beta-D-thiogalactopyranoside or lactose as an inducer.

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Bibliographic Details
Published in:Applied and environmental microbiology 1993-09, Vol.59 (9), p.3070
Main Authors: Foor, rest, Morin, Nancy, Bostian, Keith A
Format: Article
Language:English
Subjects:
Bacteria
Biochemistry
Enzymes
Genes
Online Access:Get full text
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Description
Summary:The gene encoding tyrosine phenol-lyase from Erwinia herbicola was cloned into Escherichia coli, and fusions to the lac and tac promoters were constructed. The enzyme was expressed at high levels in the presence of isopropyl-beta-D-thiogalactopyranoside or lactose as an inducer.
ISSN:0099-2240
1098-5336