Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization

A new insertion sequence was isolated from Xanthomonas campestris pv. dieffenbachiae. Sequence analysis showed that this element is 1,158 bp long and has 15-bp inverted repeat ends containing two mismatches. Comparison of this sequence with sequences in data bases revealed significant homology with...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1994-01, Vol.60 (1), p.377-384
Main Authors: Berthier, Y, Thierry, D, Lemattre, M, Guesdon, J.L
Format: Article
Language:English
Subjects:
ADN
Agronomy. Soil science and plant productions
Bacteria
Bacteriology
Base Sequence
Biological and medical sciences
Cellular biology
Deoxyribonucleic acid
DNA
DNA Primers - genetics
DNA Probes
DNA Transposable Elements
DNA, Bacterial - genetics
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetics
IDENTIFICACION
IDENTIFICATION
Life Sciences
Microbiology
Molecular Sequence Data
Plants - microbiology
POLIMORFISMO
Polymorphism, Restriction Fragment Length
POLYMORPHISME
Pseudomonadaceae - genetics
Pseudomonadaceae - isolation & purification
SECUENCIA NUCLEICA
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEIQUE
Species Specificity
XANTHOMONAS CAMPESTRIS
Xanthomonas campestris - classification
Xanthomonas campestris - genetics
Xanthomonas campestris - isolation & purification
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Description
Summary:A new insertion sequence was isolated from Xanthomonas campestris pv. dieffenbachiae. Sequence analysis showed that this element is 1,158 bp long and has 15-bp inverted repeat ends containing two mismatches. Comparison of this sequence with sequences in data bases revealed significant homology with Escherichia coli IS5. IS1051, which detected multiple restriction fragment length polymorphisms, was used as a probe to characterize strains from the pathovar dieffenbachiae
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.60.1.377-384.1994