Distribution of Pseudomonas syringae pathovars into twenty-three O serogroups

Serological reactions of Pseudomonas syringae and Pseudomonas viridiflava were studied by Ouchterlony double diffusion. A total of 55 polyclonal antisera, containing anti-lipopolysaccharide (anti-LPS) precipitating antibodies, were cross-tested against antigenic suspensions of 51 strains. Twenty-thr...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1996-07, Vol.62 (7), p.2360-2374
Main Authors: Saunier, M. (Institut National de la Recherche Agronomique, Beaucouze, France.), Malandrin, L, Samson, R
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial
ANTIGENE
ANTIGENOS
Antigens, Bacterial
Bacteria
Bacteriology
Biological and medical sciences
CLASIFICACION
CLASSIFICATION
Cross Reactions
Ecology, environment
Flowers & plants
Fundamental and applied biological sciences. Psychology
IDENTIFICACION
IDENTIFICATION
Immunoblotting
Immunodiffusion
IMMUNOLOGIE
IMMUNSERUM
INMUNOLOGIA
Life Sciences
LIPOPOLISACARIDOS
LIPOPOLYSACCHARIDE
Lipopolysaccharides - immunology
Microbiology
O Antigens - classification
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
PATHOTYPE
PATOTIPOS
Plants - microbiology
Pseudomonas - classification
Pseudomonas - immunology
Pseudomonas - pathogenicity
PSEUDOMONAS SYRINGAE
Rabbits
REACCION ANTIGENO-ANTICUERPO
REACTION ANTIGENE ANTICORPS
SEROTIPOS
SEROTYPE
Serotyping - methods
Species Specificity
SUERO INMUNE
TAXONOMIA
TAXONOMIE
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Summary:Serological reactions of Pseudomonas syringae and Pseudomonas viridiflava were studied by Ouchterlony double diffusion. A total of 55 polyclonal antisera, containing anti-lipopolysaccharide (anti-LPS) precipitating antibodies, were cross-tested against antigenic suspensions of 51 strains. Twenty-three O serogroups were defined, primarily on the reaction of the type strains. Two families of O serogroups showed antigenic cross-reactivities (PHA, MOP1, MOP2, MOP3, HEL1, HEL2, and SYR1: PERSAVTOM1, PERSAVTOM2, DEL, POR, and SYR2). Ten O serogroups showed a clearcut specificity: APTPIS, TAB, VIR1, VIR2, VIR3, SYR3, SYR4, SYR5, HUS, and LAC. The last serogroup (RIB) contained strains with rough colony morphology and side chain-deficient LPSs, as evidenced by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The LPS basis of the O serogroups was demonstrated by immunoblotting. Serological reference strains were designated for all of the O serogroups, and correspondence was established between the O serogroups studied and seven previous serogroups (L.T. Pastushenko and I.D. Simonovich, Mikrobiol. Zh. 41:222-229 and 330-339, 1979). A total of 355 strains of P. syringae (sensu lato) belonging to 15 pathovars, not including pathovar syringae, were typed into the 23 described O serogroups. O serogroups were assigned after double-diffusion reactions, with each strain compared with serological references. The utility of O serogrouping to study P. syringae pathovar structure and diversity is discussed
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.62.7.2360-2374.1996