Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes

The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high homology among the three species examined.

Saved in:
Bibliographic Details
Published in:Applied and environmental microbiology 1996-05, Vol.62 (5), p.1685
Main Authors: Rijpens, Nancy P, Jannes, Geert, Van Asbroeck, Marina, Rossau, Rudi, Herman, Lieve M F
Format: Article
Language:English
Subjects:
Bacteria
Cellular biology
Milk
Ribonucleic acid
RNA
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 5
container_start_page 1685
container_title Applied and environmental microbiology
container_volume 62
creator Rijpens, Nancy P
Jannes, Geert
Van Asbroeck, Marina
Rossau, Rudi
Herman, Lieve M F
description The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high homology among the three species examined.
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_205936168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>9708473</sourcerecordid><originalsourceid>FETCH-proquest_journals_2059361683</originalsourceid><addsrcrecordid>eNqNjMuOgkAQRTtGE3H0HyruMQWtHViOr7gyBmdvGihjK9NgNWj06yXGD3B1Fuee2xFegHHkz6RUXeEhxrEfhlPsi4FzZ0Scooo8YZeGKashp7qFKS2UR5hzk1FRaHBVNQFjgfUd_k1xgfQBu0UC2ubAdCN2BKdHyiY3T_2u76Y-QaD2fij3wMn2t_3QGTFUXKbkhqJ31IWj0Yc_Yrxe_S02fquvDbn6cC4btq06hDiLpQpUJL8avQCv2UjO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205936168</pqid></control><display><type>article</type><title>Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes</title><source>American Society for Microbiology</source><source>PubMed (Medline)</source><creator>Rijpens, Nancy P ; Jannes, Geert ; Van Asbroeck, Marina ; Rossau, Rudi ; Herman, Lieve M F</creator><creatorcontrib>Rijpens, Nancy P ; Jannes, Geert ; Van Asbroeck, Marina ; Rossau, Rudi ; Herman, Lieve M F</creatorcontrib><description>The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high homology among the three species examined.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington: American Society for Microbiology</publisher><subject>Bacteria ; Cellular biology ; Milk ; Ribonucleic acid ; RNA</subject><ispartof>Applied and environmental microbiology, 1996-05, Vol.62 (5), p.1685</ispartof><rights>Copyright American Society for Microbiology May 1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Rijpens, Nancy P</creatorcontrib><creatorcontrib>Jannes, Geert</creatorcontrib><creatorcontrib>Van Asbroeck, Marina</creatorcontrib><creatorcontrib>Rossau, Rudi</creatorcontrib><creatorcontrib>Herman, Lieve M F</creatorcontrib><title>Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes</title><title>Applied and environmental microbiology</title><description>The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high homology among the three species examined.</description><subject>Bacteria</subject><subject>Cellular biology</subject><subject>Milk</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqNjMuOgkAQRTtGE3H0HyruMQWtHViOr7gyBmdvGihjK9NgNWj06yXGD3B1Fuee2xFegHHkz6RUXeEhxrEfhlPsi4FzZ0Scooo8YZeGKashp7qFKS2UR5hzk1FRaHBVNQFjgfUd_k1xgfQBu0UC2ubAdCN2BKdHyiY3T_2u76Y-QaD2fij3wMn2t_3QGTFUXKbkhqJ31IWj0Yc_Yrxe_S02fquvDbn6cC4btq06hDiLpQpUJL8avQCv2UjO</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Rijpens, Nancy P</creator><creator>Jannes, Geert</creator><creator>Van Asbroeck, Marina</creator><creator>Rossau, Rudi</creator><creator>Herman, Lieve M F</creator><general>American Society for Microbiology</general><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>19960501</creationdate><title>Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes</title><author>Rijpens, Nancy P ; Jannes, Geert ; Van Asbroeck, Marina ; Rossau, Rudi ; Herman, Lieve M F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_2059361683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Bacteria</topic><topic>Cellular biology</topic><topic>Milk</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rijpens, Nancy P</creatorcontrib><creatorcontrib>Jannes, Geert</creatorcontrib><creatorcontrib>Van Asbroeck, Marina</creatorcontrib><creatorcontrib>Rossau, Rudi</creatorcontrib><creatorcontrib>Herman, Lieve M F</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rijpens, Nancy P</au><au>Jannes, Geert</au><au>Van Asbroeck, Marina</au><au>Rossau, Rudi</au><au>Herman, Lieve M F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes</atitle><jtitle>Applied and environmental microbiology</jtitle><date>1996-05-01</date><risdate>1996</risdate><volume>62</volume><issue>5</issue><spage>1685</spage><pages>1685-</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The 16S-23S rRNA spacer regions of Brucella abortus, B. melitensis and B. suis were cloned and subcloned after PCR amplification. Sequence analysis of the inserts revealed a spacer of about 800 bp with very high homology among the three species examined.</abstract><cop>Washington</cop><pub>American Society for Microbiology</pub></addata></record>
fulltext fulltext
identifier ISSN: 0099-2240
ispartof Applied and environmental microbiology, 1996-05, Vol.62 (5), p.1685
issn 0099-2240
1098-5336
language eng
recordid cdi_proquest_journals_205936168
source American Society for Microbiology; PubMed (Medline)
subjects Bacteria
Cellular biology
Milk
Ribonucleic acid
RNA
title Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T23%3A31%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20detection%20of%20Brucella%20spp.%20in%20raw%20milk%20by%20PCR%20and%20reverse%20hybridization%20with%2016S-23S%20rRNA%20spacer%20probes&rft.jtitle=Applied%20and%20environmental%20microbiology&rft.au=Rijpens,%20Nancy%20P&rft.date=1996-05-01&rft.volume=62&rft.issue=5&rft.spage=1685&rft.pages=1685-&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/&rft_dat=%3Cproquest%3E9708473%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-proquest_journals_2059361683%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=205936168&rft_id=info:pmid/&rfr_iscdi=true