Molecular microbial diversity in soils from eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation

Although the Amazon Basin is well known for its diversity of flora and fauna, this report represents the first description of the microbial diversity in Amazonian soils involving a culture-independent approach. Among the 100 sequences of genes coding for small-subunit rRNA obtained by PCR amplificat...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1997-07, Vol.63 (7), p.2647-2653
Main Authors: Borneman, J. (University of Wisconsin, Madison, WI.), Triplett, E.W
Format: Article
Language:English
Subjects:
ADN
AMAZONIA
AMAZONIE
Animal, plant and microbial ecology
BACTERIA
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Base Sequence
Biological and medical sciences
Brazil
Deforestation
DNA, Bacterial - analysis
FLORA DEL SUELO
FLORE DU SOL
Forestry
Fundamental and applied biological sciences. Psychology
Gene Library
Genetic Variation
Microbial ecology
Microbiology
Molecular Sequence Data
Molecular Structure
Phylogeny
Polymerase Chain Reaction - methods
RIBOSOMAS
RIBOSOME
RNA, Ribosomal - chemistry
RNA, Ribosomal - genetics
SECUENCIA NUCLEOTIDICA
Sequence Analysis, DNA
SEQUENCE NUCLEOTIDIQUE
Soil
Soil Microbiology
Soils
SOL TROPICAL
SUELO TROPICAL
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Description
Summary:Although the Amazon Basin is well known for its diversity of flora and fauna, this report represents the first description of the microbial diversity in Amazonian soils involving a culture-independent approach. Among the 100 sequences of genes coding for small-subunit rRNA obtained by PCR amplification with universal small-subunit rRNA primers, 98 were bacterial and 2 were archaeal. No duplicate sequences were found, and none of the sequences had been previously described. Eighteen percent of the bacterial sequences could not be classified in any known bacterial kingdom. Two sequences may represent a unique branch between the vast majority of bacteria and the deeply branching, predominantly thermophilic bacteria. Five sequences formed a clade that may represent a novel group within the class Proteobacteria. In addition, rRNA intergenic spacer analysis was used to show significant microbial population differences between a mature forest soil and an adjacent pasture soil
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.63.7.2647-2653.1997