Production of Recombinant Proteins in the Ion-Deficient BL21(DE3) Strain of Escherichia coli in the Absence of the DnaK Chaperone

To eliminate unavoidable contamination of purified recombinant proteins by DnaK, we present a unique approach employing a BL21(DE3) ...dnaK strain of Escherichia coli. Selected representative purified proteins remained soluble, correctly assembled, and active. This finding establishes DnaK dispensab...

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Bibliographic Details
Published in:Applied and environmental microbiology 2009-06, Vol.75 (11), p.3803
Main Authors: Ratelade, Julien, Miot, Marie-Caroline, Johnson, Emmett, Betton, Jean-Michel, Mazodier, Philippe, Benaroudj, Nadia
Format: Article
Language:English
Subjects:
Bacterial proteins
E coli
Microbiology
Proteases
Online Access:Get full text
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Summary:To eliminate unavoidable contamination of purified recombinant proteins by DnaK, we present a unique approach employing a BL21(DE3) ...dnaK strain of Escherichia coli. Selected representative purified proteins remained soluble, correctly assembled, and active. This finding establishes DnaK dispensability for protein production in BL21(DE3), which is void of Lon protease, key to eliminating unfolded proteins. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:0099-2240
1098-5336