Production of Recombinant Proteins in the Ion-Deficient BL21(DE3) Strain of Escherichia coli in the Absence of the DnaK Chaperone

To eliminate unavoidable contamination of purified recombinant proteins by DnaK, we present a unique approach employing a BL21(DE3) ...dnaK strain of Escherichia coli. Selected representative purified proteins remained soluble, correctly assembled, and active. This finding establishes DnaK dispensab...

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Published in:Applied and environmental microbiology 2009-06, Vol.75 (11), p.3803
Main Authors: Ratelade, Julien, Miot, Marie-Caroline, Johnson, Emmett, Betton, Jean-Michel, Mazodier, Philippe, Benaroudj, Nadia
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Language:English
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Bacterial proteins
E coli
Microbiology
Proteases
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container_issue 11
container_start_page 3803
container_title Applied and environmental microbiology
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creator Ratelade, Julien
Miot, Marie-Caroline
Johnson, Emmett
Betton, Jean-Michel
Mazodier, Philippe
Benaroudj, Nadia
description To eliminate unavoidable contamination of purified recombinant proteins by DnaK, we present a unique approach employing a BL21(DE3) ...dnaK strain of Escherichia coli. Selected representative purified proteins remained soluble, correctly assembled, and active. This finding establishes DnaK dispensability for protein production in BL21(DE3), which is void of Lon protease, key to eliminating unfolded proteins. (ProQuest: ... denotes formulae/symbols omitted.)
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source Open Access: PubMed Central; American Society for Microbiology Journals
subjects Bacterial proteins
E coli
Microbiology
Proteases
title Production of Recombinant Proteins in the Ion-Deficient BL21(DE3) Strain of Escherichia coli in the Absence of the DnaK Chaperone
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