Accurate Quantification of Microorganisms in PCR-Inhibiting Environmental DNA Extracts by a Novel Internal Amplification Control Approach Using Biotrove OpenArrays

• Published in: Applied and Environmental Microbiology 2009-11, Vol.75 (22), p.7253-7260
• Main Authors: VAN DOORN, R, KLERKS, M. M, VAN GENT-PELZER, M. P. E, SPEKSNIJDER, A. G. C. L, KOWALCHUK, G. A, SCHOEN, C. D
• Format: Article
• Language: English
• Subjects: assays; Biological and medical sciences; Calibration; Deoxyribonucleic acid; diagnostic pcr; DNA; DNA, Bacterial - analysis; DNA, Bacterial - genetics; Environment; Environmental Microbiology; Environmental Monitoring - methods; Environmental science; Fundamental and applied biological sciences. Psychology; Genes; Genetics; humic substances; Methods; Microarray Analysis - methods; Microbiology; Microorganisms; Molecular Sequence Data; Polymerase Chain Reaction - methods; polymerase-chain-reaction; positive control; purification; quantitative pcr; real-time pcr; Reference Standards; Reproducibility of Results; salmonella-enterica; Sensitivity and Specificity; soil
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.00796-09

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