Molecular and metabolic characterization of cold-tolerant alpine soil Pseudomonas sensu stricto

Alpine soils undergo dramatic temporal changes in their microclimatic properties, suggesting that the bacteria there encounter uncommon shifting selection gradients. Pseudomonads constitute important members of the alpine soil community. In order to characterize the alpine Pseudomonas community and...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2004, Vol.70 (1), p.483-489
Main Authors: Meyer, A.F, Lipson, D.A, Martin, A.P, Schadt, C.W, Schmidt, S.K
Format: Article
Language:English
Subjects:
alpine meadow soils
Altitude
Bacteria
Bacterial Typing Techniques
Biological and medical sciences
Climate change
cold soils
Cold Temperature
cold tolerance
Colony Count, Microbial
Colorado
Culture Media
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Fundamental and applied biological sciences. Psychology
Metabolism
Microbial Ecology
microbial physiology
Microbiology
Molecular Sequence Data
mountain soils
nucleotide sequences
phylogeny
Polymerase Chain Reaction
Pseudomonas
Pseudomonas - classification
Pseudomonas - genetics
Pseudomonas - growth & development
Pseudomonas - metabolism
ribosomal DNA
RNA, Ribosomal, 16S - genetics
sequence alignment
Sequence Analysis, DNA
soil bacteria
Soil Microbiology
Soils
tundra soils
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Summary:Alpine soils undergo dramatic temporal changes in their microclimatic properties, suggesting that the bacteria there encounter uncommon shifting selection gradients. Pseudomonads constitute important members of the alpine soil community. In order to characterize the alpine Pseudomonas community and to assess the impact of shifting selection on this community, we examined the ability of cold-tolerant Pseudomonas isolates to grow on a variety of carbon sources, and we determined their phylogenetic relationships based on 16S ribosomal DNA sequencing. We found a high prevalence of Pseudomonas in our soil samples, and isolates from these soils exhibited extensive metabolic diversity. In addition, our data revealed that many of our isolates form a unique cold-adapted clade, representatives of which are also found in the Swedish tundra and Antarctica. Our data also show a lack of concordance between the metabolic properties and 16S phylogeny, indicating that the metabolic diversity of these organisms cannot be predicted by phylogeny.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.70.1.483-489.2004