Effect of Growth Phase Feeding Strategies on Succinate Production by Metabolically Engineered Escherichia coli

Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cy...

Full description

Saved in:
Bibliographic Details
Published in:Applied and Environmental Microbiology 2010-02, Vol.76 (4), p.1298-1300
Main Authors: Jiang, Min, Liu, Shu-wen, Ma, Jiang-feng, Chen, Ke-quan, Yu, Li, Yue, Fang-fang, Xu, Bing, Wei, Ping
Format: Article
Language:English
Subjects:
Aerobiosis
Anaerobiosis
Bacterial Proteins - genetics
Biological and medical sciences
Biotechnology
Cell growth
Citric Acid Cycle
E coli
Enzymes
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Gene Deletion
Gene expression
Genes, Bacterial
Genetic Engineering
Glucose
Glucose - metabolism
Glyoxylates - metabolism
Microbiology
Mutation
Oxidation-Reduction
Phosphoenolpyruvate Sugar Phosphotransferase System - genetics
Sigma Factor - genetics
Succinic Acid - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.02190-09