Cloning and Expression of Two Crystal Protein Genes, cry30Ba1 and cry44Aa1, Obtained from a Highly Mosquitocidal Strain, Bacillus thuringiensis subsp. entomocidus INA288

Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC₅₀] = 6 ng/ml) and Aedes aegy...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2006-08, Vol.72 (8), p.5673-5676
Main Authors: Ito, Takeshi, Ikeya, Tomonori, Sahara, Ken, Bando, Hisanori, Asano, Shin-ichiro
Format: Article
Language:English
Subjects:
Aedes - drug effects
Aedes aegypti
Animals
Bacillus thuringiensis
Bacillus thuringiensis - genetics
Bacillus thuringiensis - metabolism
Bacillus thuringiensis - pathogenicity
Bacillus thuringiensis subsp. entomocidus
bacterial insecticides
Bacterial proteins
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacterial Proteins - pharmacology
Bacterial Toxins - genetics
Bacterial Toxins - metabolism
Bacterial Toxins - pharmacology
Bacteriology
Cloning
Cloning, Molecular
cry30Ba gene
cry44Aa gene
crystal proteins
Culex - drug effects
Culex pipiens pallens
Endotoxins - genetics
Endotoxins - metabolism
Endotoxins - pharmacology
entomopathogenic bacteria
gene expression
Genes
Hemolysin Proteins
insecticidal properties
insecticidal proteins
Invertebrate Microbiology
Molecular Sequence Data
nucleotide sequences
Sequence Analysis, DNA
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Description
Summary:Two novel crystal protein genes, cry30Ba and cry44Aa, were cloned from Bacillus thuringiensis subsp. entomocidus INA288 and expressed in an acrystalliferous strain. Cry44Aa crystals were highly toxic to second-instar Culex pipiens pallens (50% mortality concentration [LC₅₀] = 6 ng/ml) and Aedes aegypti (LC₅₀ = 12 ng/ml); however, Cry30Ba crystals were not toxic.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.01894-05