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POSSIBLE EFFECT OF CHOLESTERYL-ESTER TRANSFER PROTEIN ACTIVITY ON PARAOXONASE 1 ANTIOXIDANT FUNCTION

Background and objectives: Paraoxonase 1 (PON1) is a glycoprotein enzyme which is mainly carried by high density lipoproteins (HDL) in plasma. This enzyme has been proposed as one of the major contributors to HDL antioxidant activity. Its functionality is highly dependent on apolipoprotein (apo) A-I...

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Bibliographic Details
Published in:Annals of nutrition and metabolism 2017-10, Vol.71 (Suppl. 2), p.477
Main Authors: Botta, Eliana Elizabeth, Verona, Julian, Martin, Maximiliano E, Gilligan, Lisandro E, Tetzlaff, Walter Francisco, Boero, Laura, Brites, Fernando D
Format: Article
Language:English
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Summary:Background and objectives: Paraoxonase 1 (PON1) is a glycoprotein enzyme which is mainly carried by high density lipoproteins (HDL) in plasma. This enzyme has been proposed as one of the major contributors to HDL antioxidant activity. Its functionality is highly dependent on apolipoprotein (apo) A-I-mediated stabilization within the HDL particle. A previous study by our group showed a decrease in PON1 antioxidant capacity in hypertriglyceridemia. CETP is a plasma protein responsible for the exchange of cholesteryl-esters and triglycerides (TG) between HDL and apo B-containing lipoproteins, usually resulting in an increase in HDL TG content. Furthermore, TG enrichment of HDL has been shown to lead to particle remodeling and even loss of apo A-I. Objective: To evaluate the effect of CETP activity on PON1 status. Methods: 150 children between 7 and 14 years old were recruited from the city of Balcarce, Argentina. Anthropometric parameters were registered and body mass index was calculated (BMI). Glucose levels and lipid profile were evaluated by automatized methods. PON1 was measured employing paraoxon (PON activity) and phenylacetate (ARE activity) as substrates, given that PON activity better reflects the intrinsic antioxidant activity and ARE activity better correlates with the enzymatic concentration. CETP activity was quantified by a radiometric assay. Results: The population as a whole was normoglycemic, normotrycliceridemic and normocholesterolemic. In univariate association analysis PON1 correlated with ARE activity (r=0.4; p
ISSN:0250-6807
1421-9697
DOI:10.1159/000480486