INVESTIGATION OF BLOOD ANTIOXIDANT ENZYME LEVELS AND GLUTATHIONE PEROXIDASE, CATALASE, AND SUPEROXIDE DISMUTASE GENE POLYMORPHISM IN SHEEP WITH FLUOROSIS

Reactive oxygen species have been considered to play a significant part in the development of fluorosis, a critical public health issue in many parts of the world. OBJECTIVES: The aims of this study, conducted on two groups of sheep, with and without fluorosis, was (i) to determine the effect of flu...

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Bibliographic Details
Published in:Fluoride 2017-07, Vol.50 (3), p.374-382
Main Authors: Ozbey, Ulku, Deger, Yeter, Yur, Fatmagul, Çambay, Zafer, Ozbey, Gokben
Format: Article
Language:English
Subjects:
Animal sciences
Animals
Antioxidants
Deoxyribonucleic acid
DNA
Enzymes
Fluorides
Free radicals
Genotype & phenotype
Investigations
Lipid peroxidation
Lipids
Methods
Migraine
Oxidative stress
Polymerase chain reaction
Public health
Rodents
Sheep
Urine
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Summary:Reactive oxygen species have been considered to play a significant part in the development of fluorosis, a critical public health issue in many parts of the world. OBJECTIVES: The aims of this study, conducted on two groups of sheep, with and without fluorosis, was (i) to determine the effect of fluorosis on antioxidant enzymes, such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and on malondialdehyde (MDA); and (ii) to investigate the relationship between the SOD, CAT, and GSH-Px genotypes and the antioxidant enzyme levels. METHODS: The two groups of sheep were: (i) 24 Akkaraman sheep with fluorosis living in Doğubeyazit province of Ağrı and (ii) 20 Akkaraman sheep with no signs of fluorosis living in Van. The MDA level and the antioxidant enzyme activities were identified spectrophometrically. The genotype characteristics were determined by the PCR-RFLP method using DNA extracted from blood. RESULTS: Compared to the control sheep without fluorosis, we found in the fluorotic sheep: (i) an increase in plasma MDA levels and GSH-Px-1 activity (p
ISSN:0015-4725
2253-4083