A long-wavelength fluorescent probe for amino compounds and its application in the determination of aliphatic amines

Herein, a highly reactive long-wavelength fluorescent labeling reagent, 1,7-dimethyl-3,5-distyryl-8-phenyl-(4-oxy-acetic acid N -hydroxysuccinimidyl ester) difluoroboradiaza- s -indacene (DMDSPAB-OSu), was designed and synthesized for amino compounds. Using DMDSPAB-OSu as a pre-column derivatization...

Full description

Saved in:
Bibliographic Details
Published in:Analytical methods 2018-01, Vol.10 (26), p.3188-3196
Main Authors: Xia, Li-Jun, Guo, Xiao-Feng, Ji, Yan, Chen, Liu, Wang, Hong
Format: Article
Language:English
Subjects:
Acetic acid
Aliphatic amines
Amines
Fluorescence
Fluorescent indicators
High-performance liquid chromatography
Liquid chromatography
Reagents
Urine
Wavelength
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Herein, a highly reactive long-wavelength fluorescent labeling reagent, 1,7-dimethyl-3,5-distyryl-8-phenyl-(4-oxy-acetic acid N -hydroxysuccinimidyl ester) difluoroboradiaza- s -indacene (DMDSPAB-OSu), was designed and synthesized for amino compounds. Using DMDSPAB-OSu as a pre-column derivatization reagent, an HPLC-fluorescence detection method was developed for the determination of trace amounts of aliphatic amines in samples. Prior to analysis, amines were derivatized with DMDSPAB-OSu at room temperature in 0.2 mol L −1 pH 8.8 borate buffer for only 5 min, and the baseline separation of 11 aliphatic amines was achieved within 25 min. Limits of detection between 0.8 to 1.2 nM were obtained at 630 nm with an excitation wavelength of 610 nm. Furthermore, the proposed DMDSPAB-OSu-based HPLC method was applied to the analysis of lake water and human urine with satisfying recoveries ranging from 93.0–109.7%.
ISSN:1759-9660
1759-9679
DOI:10.1039/C8AY00706C