Inkjet 3D printed chip for capillary gel electrophoresis

• Published in: Sensors and actuators. B, Chemical Chemical, 2018-05, Vol.261, p.474-480
• Main Authors: Walczak, Rafał, Adamski, Krzysztof, Kubicki, Wojciech
• Format: Article
• Language: English
• Subjects: Capillary electrophoresis; Configurations; Construction materials; Deoxyribonucleic acid; DNA; Electrophoresis; Fluorescence; Gel electrophoresis; Ink jet printing; Inkjet 3D printing; Inkjet printing; Lab-on-a-chip; Microchannels; Optical properties; Separation; Studies; Surface roughness; Three dimensional printing
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2018.01.174

•We presented for the first time inkjet 3D printed chip for on-chip gel electrophoresis.•We found that printing orientation of the microchannels plays a crucial role in the proper operation of the separation process.•The building material used in this study is semi-transparent and autofluorescence of the material is not noticed, thus fluorescence detection is possible.•50–800 bp DNA ladder can be separated in the printed chip with fluorescence detection at the end of the separation microchannel. This paper presents for the first time the use of an inkjet 3D printing to develop a chip for capillary gel electrophoresis. The designing of the chip is preceded by investigations into surface roughness and the geometrical properties of the printed microchannels, which are important considerations for the separation process. The optical properties of the building material are also determined to confirm whether fluorescence detection is possible. It is found that the printing orientation of the microchannels plays a crucial role in the proper operation of the separation process. A description of the successful separation of a 50–800 bp DNA ladder, which was achieved with fluorescence detection at the end of separation microchannel, is given. Based on electropherograms, the number of theoretical separation plates is calculated (max. 70,000) and compared with data from the literature. Finally, some conclusions and observations on the design of the chip are given in order to improve its configuration in the future toward modular configuration.