Electrochemicolor imaging of endogenous alkaline phosphatase and respiratory activities of mesenchymal stem cell aggregates in early-stage osteodifferentiation

Mesenchymal stem cells (MSCs) have received great attention for tissue engineering. In the present study, the early stage of osteogenesis of MSC aggregates was monitored by electrochemicolor imaging using an electrode array device that can simultaneously detect multiple redox species. Electrochemico...

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Bibliographic Details
Published in:Electrochimica acta 2018-04, Vol.268, p.554-561
Main Authors: Ino, Kosuke, Onodera, Takehiro, Kanno, Yusuke, Suda, Atsushi, Kunikata, Ryota, Matsue, Tomokazu, Shiku, Hitoshi
Format: Article
Language:English
Subjects:
Aggregates
Alkaline phosphatase
Biocompatibility
Bone diseases
Cell analysis
Cellular biology
Electrochemical bioimaging
Electrode array device
Electrodes
Hydroxyapatite
Imaging
Medical treatment
Mesenchymal stem cells
Osteogenesis
Stem cells
Tissue engineering
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Summary:Mesenchymal stem cells (MSCs) have received great attention for tissue engineering. In the present study, the early stage of osteogenesis of MSC aggregates was monitored by electrochemicolor imaging using an electrode array device that can simultaneously detect multiple redox species. Electrochemicolor imaging was used to evaluate the cells' endogenous alkaline phosphatase (ALP) and respiratory activities. High ALP activity was observed in the aggregates after osteomedium (OM) treatment, with hydroxyapatite addition inducing early-stage osteogenesis in large aggregates. In small aggregates, OM treatment decreased the respiratory activity. To the best of our knowledge, this is the first report to use electrochemical imaging of ALP and respiratory activities to evaluate osteogenesis in MSC aggregates.
ISSN:0013-4686
1873-3859
DOI:10.1016/j.electacta.2018.02.094