A near-infrared lysosomal pH probe based on rhodamine derivative

A near-infrared fluorescent probe for pH was designed based on the rhodamine derivative, which responded to pH with rapid response, high sensitivity and desirable anti-interference ability. The probe was also applied to target lysosomes and monitor lysosomal pH variations successfully. [Display omit...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-03, Vol.256, p.261-267
Main Authors: Shen, Shi-Li, Zhang, Xiao-Fan, Ge, Yan-Qing, Zhu, Yan, Lang, Xue-Qin, Cao, Xiao-Qun
Format: Article
Language:English
Subjects:
Apoptosis
Cell growth
Derivatives
Fluorescence
Fluorescent indicators
Fluorescent probe
Infrared radiation
Intracellular pH
Lysosomes
Morpholine
Near-infrared
Response time
Rhodamine
Rhodamine derivative
Target detection
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Summary:A near-infrared fluorescent probe for pH was designed based on the rhodamine derivative, which responded to pH with rapid response, high sensitivity and desirable anti-interference ability. The probe was also applied to target lysosomes and monitor lysosomal pH variations successfully. [Display omitted] •A new fluorescent pH probe that emitted in NIR region was synthesized based on rhodamine derivative.•The probe with a pKa of 4.98 was suitable for studying acidic organelles in living cells.•The probe responded to pH with high sensitivity, rapid response and excellent anti-interference capability.•The probe could selectively stain lysosomes with low cytotoxicity. A near-infrared fluorescent probe (NRL) for lysosomal pH was presented by integrating 4-(2-aminoethyl)morpholine moiety, a lysosome-targetable group, into the rhodamine derivative. The addition of H+ led to significant fluorescence intensity increase at 739nm, thus enabling it to monitor pH changes at weakly acidic pH. NRL showed negligible response to common species and short response time. Furthermore, the probe was successfully applied in living cells, and the results indicated that the probe could target lysosomes and detect lysosomal pH. We believe that NRL had great potential as a practical tool for lysosomal pH biology.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2017.10.103