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A lateral flow channel immunoassay combining a particle binding zone geometry with nanoparticle labelling amplification

•Channel flow design with reaction area: sample volume >20 times the open channel immunoassay format.•Simple channel flow design for samples of ≥10 μL.•Reaction zone of silica particles with covalently linked capture antibodies, combined with non-direct fluorescent nanoparticles.•Strong fluoresce...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-06, Vol.262, p.1-8
Main Authors: Engels, Jan F., Henderson, Cassi J., Daly, Ronan, Renneberg, Reinhard, Hall, Elizabeth A.H.
Format: Article
Language:English
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Summary:•Channel flow design with reaction area: sample volume >20 times the open channel immunoassay format.•Simple channel flow design for samples of ≥10 μL.•Reaction zone of silica particles with covalently linked capture antibodies, combined with non-direct fluorescent nanoparticles.•Strong fluorescent signal generated from fluorescent pre-cursor particles.•Model system of RbIgG/Gt-α RbIgG showed good linearity for detection of
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2018.01.213