Ameliorative Effects of Selenium on Cadmium-Induced Injury in the Chicken Ovary: Mechanisms of Oxidative Stress and Endoplasmic Reticulum Stress in Cadmium-Induced Apoptosis

• Published in: Biological trace element research 2018-08, Vol.184 (2), p.463-473
• Main Authors: Wan, Na, Xu, Zhe, Liu, Tianqi, Min, Yahong, Li, Shu
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Activating Transcription Factor 4 - genetics; Activating Transcription Factor 4 - metabolism; Animal tissues; Animals; Apoptosis; Apoptosis - drug effects; Basic diets; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cadmium; Cadmium - administration & dosage; Cadmium - toxicity; Cadmium chloride; Chickens; Diet; Dietary Supplements; Endoplasmic reticulum; Endoplasmic Reticulum Stress - drug effects; Estrogen receptors; Female; Females; Gene Expression - drug effects; Life Sciences; Nitric Oxide Synthase Type II - genetics; Nitric Oxide Synthase Type II - metabolism; Nutrition; Oncology; Organ Size - drug effects; Ovaries; Ovary - drug effects; Ovary - metabolism; Ovary - pathology; Oxidative stress; Oxidative Stress - drug effects; Poultry; Random Allocation; Selenium; Selenium - administration & dosage; Selenium - pharmacology; Sex hormones; Sodium; Sodium selenite; Tissue; Toxicity
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-017-1193-x

Despite the well-established toxicity of cadmium (Cd) to animals and the ameliorative effects of selenium (Se), some specific mechanisms in the chicken ovary are not yet clarified. To explore the mechanism by which the toxicity effect of Cd is induced and explore the effect of supranutritional Se on Cd toxicity in female bird reproduction, forty-eight 50-day-old Isa Brown female chickens were divided randomly into four groups. Group I (control group) was fed the basic diet containing 0.2 mg/kg Se. Group II (Se-treated group) was fed the basic diet supplemented with sodium selenite (Na 2 SeO 3 ), and the total Se content was 2 mg/kg. Group III (Se + Cd-treated group) was fed the basic diet supplemented with Na 2 SeO 3 ; the total Se content was 2 mg/kg, and it was supplemented with 150 mg/kg cadmium chloride (CdCl 2 ). Group IV (Cd-treated group) was with the basic diet supplemented with 150 mg/kg CdCl 2 . The Cd, estradiol (E2), and progestogen (P4) contents changed after subchronic Cd exposure in chicken ovarian tissue; subsequently, oxidative stress occurred and activated the endoplasmic reticulum (ER) pathway to induce apoptosis. Further, Se decreased the accumulation of Cd in ovarian tissue, increased the E2 and P4 contents, alleviated oxidative stress, and reduced apoptosis via the ER stress pathway. The present results demonstrated that Cd could induce apoptosis via the ER stress pathway in chicken ovarian tissue and that Se had a significant antagonistic effect. These results are potentially valuable for finding a strategy to prevent Cd poisoning.