Plasma membrane ion permeability induced by mutant [alpha]-synuclein contributes to the degeneration of neural cells

Mutations in alpha-synuclein cause some cases of familial Parkinson's disease (PD), but the mechanism by which alpha-synuclein promotes degeneration of dopamine-producing neurons is unknown. We report that human neural cells expressing mutant alpha-synuclein (A30P and A53T) have higher plasma m...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neurochemistry 2006-05, Vol.97 (4), p.1071
Main Authors: Furukawa, Katsutoshi, Matsuzaki-Kobayashi, Michiko, Hasegawa, Takafumi, Kikuchi, Akio, Sugeno, Naoto, Itoyama, Yasuto, Wang, Yue, Yao, Pamela J, Bushlin, Ittai, Takeda, Atsushi
Format: Article
Language:English
Subjects:
Ions
Membranes
Mutation
Neurons
Parkinson's disease
Permeability
Plasma
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page
container_issue 4
container_start_page 1071
container_title Journal of neurochemistry
container_volume 97
creator Furukawa, Katsutoshi
Matsuzaki-Kobayashi, Michiko
Hasegawa, Takafumi
Kikuchi, Akio
Sugeno, Naoto
Itoyama, Yasuto
Wang, Yue
Yao, Pamela J
Bushlin, Ittai
Takeda, Atsushi
description Mutations in alpha-synuclein cause some cases of familial Parkinson's disease (PD), but the mechanism by which alpha-synuclein promotes degeneration of dopamine-producing neurons is unknown. We report that human neural cells expressing mutant alpha-synuclein (A30P and A53T) have higher plasma membrane ion permeability. The higher ion permeability caused by mutant alpha-synuclein would be because of relatively large pores through which most cations can pass non-selectively. Both the basal level of [Ca2+]i and the Ca2+ response to membrane depolarization are greater in cells expressing mutant alpha-synuclein. The membrane permeable Ca2+ chelator BAPTA-AM significantly protected the cells against oxidative stress, whereas neitherl-type (nifedipine) nor N-type (omega-conotoxin-GVIA) Ca2+ channel blockers protected the cells. These findings suggest that the high membrane ion permeability caused by mutant alpha-synuclein may contribute to the degeneration of neurons in PD.[PUBLICATION ABSTRACT]
doi_str_mv 10.1111/j.1471-4159.2006.03803.x
format article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_206536084</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1027520781</sourcerecordid><originalsourceid>FETCH-proquest_journals_2065360843</originalsourceid><addsrcrecordid>eNqNjLlKBEEURQtRsF3-4WHe5atlyjEWxdDATGSo7n7jVFNLWwvYf-8IYuxJbnAuhzEQyMWR25kLfSd6LTb3XCIajmqLin-dsO5PnLIOUcpeoZbn7KKUGVEYbUTH6ou3JVgIFIZsI4FLERbKgezgvKsruDi1kSYYVgit2ljhzfrlYN_7ssY2enIRxhRrdkOrVKAmqAeCiT4oUrb1J5j2EKll62Ek78sVO9tbX-j6dy_ZzdPj68Nzv-T02ajU3Zxajke1k2g2yuBWq3-dvgE6dVTa</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>206536084</pqid></control><display><type>article</type><title>Plasma membrane ion permeability induced by mutant [alpha]-synuclein contributes to the degeneration of neural cells</title><source>Full-Text Journals in Chemistry (Open access)</source><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Furukawa, Katsutoshi ; Matsuzaki-Kobayashi, Michiko ; Hasegawa, Takafumi ; Kikuchi, Akio ; Sugeno, Naoto ; Itoyama, Yasuto ; Wang, Yue ; Yao, Pamela J ; Bushlin, Ittai ; Takeda, Atsushi</creator><creatorcontrib>Furukawa, Katsutoshi ; Matsuzaki-Kobayashi, Michiko ; Hasegawa, Takafumi ; Kikuchi, Akio ; Sugeno, Naoto ; Itoyama, Yasuto ; Wang, Yue ; Yao, Pamela J ; Bushlin, Ittai ; Takeda, Atsushi</creatorcontrib><description>Mutations in alpha-synuclein cause some cases of familial Parkinson's disease (PD), but the mechanism by which alpha-synuclein promotes degeneration of dopamine-producing neurons is unknown. We report that human neural cells expressing mutant alpha-synuclein (A30P and A53T) have higher plasma membrane ion permeability. The higher ion permeability caused by mutant alpha-synuclein would be because of relatively large pores through which most cations can pass non-selectively. Both the basal level of [Ca2+]i and the Ca2+ response to membrane depolarization are greater in cells expressing mutant alpha-synuclein. The membrane permeable Ca2+ chelator BAPTA-AM significantly protected the cells against oxidative stress, whereas neitherl-type (nifedipine) nor N-type (omega-conotoxin-GVIA) Ca2+ channel blockers protected the cells. These findings suggest that the high membrane ion permeability caused by mutant alpha-synuclein may contribute to the degeneration of neurons in PD.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.2006.03803.x</identifier><language>eng</language><publisher>New York: Blackwell Publishing Ltd</publisher><subject>Ions ; Membranes ; Mutation ; Neurons ; Parkinson's disease ; Permeability ; Plasma</subject><ispartof>Journal of neurochemistry, 2006-05, Vol.97 (4), p.1071</ispartof><rights>2006 The Authors Journal Compilation 2006 International Society for Neurochemistry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Furukawa, Katsutoshi</creatorcontrib><creatorcontrib>Matsuzaki-Kobayashi, Michiko</creatorcontrib><creatorcontrib>Hasegawa, Takafumi</creatorcontrib><creatorcontrib>Kikuchi, Akio</creatorcontrib><creatorcontrib>Sugeno, Naoto</creatorcontrib><creatorcontrib>Itoyama, Yasuto</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Yao, Pamela J</creatorcontrib><creatorcontrib>Bushlin, Ittai</creatorcontrib><creatorcontrib>Takeda, Atsushi</creatorcontrib><title>Plasma membrane ion permeability induced by mutant [alpha]-synuclein contributes to the degeneration of neural cells</title><title>Journal of neurochemistry</title><description>Mutations in alpha-synuclein cause some cases of familial Parkinson's disease (PD), but the mechanism by which alpha-synuclein promotes degeneration of dopamine-producing neurons is unknown. We report that human neural cells expressing mutant alpha-synuclein (A30P and A53T) have higher plasma membrane ion permeability. The higher ion permeability caused by mutant alpha-synuclein would be because of relatively large pores through which most cations can pass non-selectively. Both the basal level of [Ca2+]i and the Ca2+ response to membrane depolarization are greater in cells expressing mutant alpha-synuclein. The membrane permeable Ca2+ chelator BAPTA-AM significantly protected the cells against oxidative stress, whereas neitherl-type (nifedipine) nor N-type (omega-conotoxin-GVIA) Ca2+ channel blockers protected the cells. These findings suggest that the high membrane ion permeability caused by mutant alpha-synuclein may contribute to the degeneration of neurons in PD.[PUBLICATION ABSTRACT]</description><subject>Ions</subject><subject>Membranes</subject><subject>Mutation</subject><subject>Neurons</subject><subject>Parkinson's disease</subject><subject>Permeability</subject><subject>Plasma</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNjLlKBEEURQtRsF3-4WHe5atlyjEWxdDATGSo7n7jVFNLWwvYf-8IYuxJbnAuhzEQyMWR25kLfSd6LTb3XCIajmqLin-dsO5PnLIOUcpeoZbn7KKUGVEYbUTH6ou3JVgIFIZsI4FLERbKgezgvKsruDi1kSYYVgit2ljhzfrlYN_7ssY2enIRxhRrdkOrVKAmqAeCiT4oUrb1J5j2EKll62Ek78sVO9tbX-j6dy_ZzdPj68Nzv-T02ajU3Zxajke1k2g2yuBWq3-dvgE6dVTa</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Furukawa, Katsutoshi</creator><creator>Matsuzaki-Kobayashi, Michiko</creator><creator>Hasegawa, Takafumi</creator><creator>Kikuchi, Akio</creator><creator>Sugeno, Naoto</creator><creator>Itoyama, Yasuto</creator><creator>Wang, Yue</creator><creator>Yao, Pamela J</creator><creator>Bushlin, Ittai</creator><creator>Takeda, Atsushi</creator><general>Blackwell Publishing Ltd</general><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20060501</creationdate><title>Plasma membrane ion permeability induced by mutant [alpha]-synuclein contributes to the degeneration of neural cells</title><author>Furukawa, Katsutoshi ; Matsuzaki-Kobayashi, Michiko ; Hasegawa, Takafumi ; Kikuchi, Akio ; Sugeno, Naoto ; Itoyama, Yasuto ; Wang, Yue ; Yao, Pamela J ; Bushlin, Ittai ; Takeda, Atsushi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_2065360843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Ions</topic><topic>Membranes</topic><topic>Mutation</topic><topic>Neurons</topic><topic>Parkinson's disease</topic><topic>Permeability</topic><topic>Plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furukawa, Katsutoshi</creatorcontrib><creatorcontrib>Matsuzaki-Kobayashi, Michiko</creatorcontrib><creatorcontrib>Hasegawa, Takafumi</creatorcontrib><creatorcontrib>Kikuchi, Akio</creatorcontrib><creatorcontrib>Sugeno, Naoto</creatorcontrib><creatorcontrib>Itoyama, Yasuto</creatorcontrib><creatorcontrib>Wang, Yue</creatorcontrib><creatorcontrib>Yao, Pamela J</creatorcontrib><creatorcontrib>Bushlin, Ittai</creatorcontrib><creatorcontrib>Takeda, Atsushi</creatorcontrib><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furukawa, Katsutoshi</au><au>Matsuzaki-Kobayashi, Michiko</au><au>Hasegawa, Takafumi</au><au>Kikuchi, Akio</au><au>Sugeno, Naoto</au><au>Itoyama, Yasuto</au><au>Wang, Yue</au><au>Yao, Pamela J</au><au>Bushlin, Ittai</au><au>Takeda, Atsushi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma membrane ion permeability induced by mutant [alpha]-synuclein contributes to the degeneration of neural cells</atitle><jtitle>Journal of neurochemistry</jtitle><date>2006-05-01</date><risdate>2006</risdate><volume>97</volume><issue>4</issue><spage>1071</spage><pages>1071-</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><abstract>Mutations in alpha-synuclein cause some cases of familial Parkinson's disease (PD), but the mechanism by which alpha-synuclein promotes degeneration of dopamine-producing neurons is unknown. We report that human neural cells expressing mutant alpha-synuclein (A30P and A53T) have higher plasma membrane ion permeability. The higher ion permeability caused by mutant alpha-synuclein would be because of relatively large pores through which most cations can pass non-selectively. Both the basal level of [Ca2+]i and the Ca2+ response to membrane depolarization are greater in cells expressing mutant alpha-synuclein. The membrane permeable Ca2+ chelator BAPTA-AM significantly protected the cells against oxidative stress, whereas neitherl-type (nifedipine) nor N-type (omega-conotoxin-GVIA) Ca2+ channel blockers protected the cells. These findings suggest that the high membrane ion permeability caused by mutant alpha-synuclein may contribute to the degeneration of neurons in PD.[PUBLICATION ABSTRACT]</abstract><cop>New York</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1471-4159.2006.03803.x</doi></addata></record>
fulltext fulltext
identifier ISSN: 0022-3042
ispartof Journal of neurochemistry, 2006-05, Vol.97 (4), p.1071
issn 0022-3042
1471-4159
language eng
recordid cdi_proquest_journals_206536084
source Full-Text Journals in Chemistry (Open access); Wiley-Blackwell Read & Publish Collection
subjects Ions
Membranes
Mutation
Neurons
Parkinson's disease
Permeability
Plasma
title Plasma membrane ion permeability induced by mutant [alpha]-synuclein contributes to the degeneration of neural cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T13%3A48%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20membrane%20ion%20permeability%20induced%20by%20mutant%20%5Balpha%5D-synuclein%20contributes%20to%20the%20degeneration%20of%20neural%20cells&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Furukawa,%20Katsutoshi&rft.date=2006-05-01&rft.volume=97&rft.issue=4&rft.spage=1071&rft.pages=1071-&rft.issn=0022-3042&rft.eissn=1471-4159&rft_id=info:doi/10.1111/j.1471-4159.2006.03803.x&rft_dat=%3Cproquest%3E1027520781%3C/proquest%3E%3Cgrp_id%3Ecdi_FETCH-proquest_journals_2065360843%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=206536084&rft_id=info:pmid/&rfr_iscdi=true