Uniform distribution of microspheres based on pressure difference for carcinoma-embryonic antigen detection

•Uniform distribution of microspheres based on pressure difference was realized.•This immunoassay system showed high sensitivity and specificity.•We simplified operations and reduced time cost via co-incubation method.•There was high consistency between our experiment results and clinical data. Micr...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2018-04, Vol.258, p.558-565
Main Authors: Wang, Kun, Zhou, Lin, Wang, Zhishuo, Cheng, Zule, Dong, Haidao, Wu, Zhenhua, Bai, Yanan, Jin, Qinghui, Mao, Hongju, Zhao, Jianlong
Format: Article
Language:English
Subjects:
Biomarkers
Biomolecules
Cancer
Immunoassay
Microfluidics
Microspheres
Quantum dots
Reagents
Sensors
Stress concentration
Trap efficiency
Tumor biomarkers
Tumors
Uniform distribution
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Summary:•Uniform distribution of microspheres based on pressure difference was realized.•This immunoassay system showed high sensitivity and specificity.•We simplified operations and reduced time cost via co-incubation method.•There was high consistency between our experiment results and clinical data. Microspheres coated with specific biomolecules have been broadly employed to detect tumor markers. In this study, we developed a novel microfluidic platform based on pressure difference for the uniform distribution of microspheres and applied this platform for carcinoma-embryonic antigen detection with quantum dots probes. Within our microfluidic device, micropost arrays were designed to ensure that the trapped microspheres formed dot arrays to overcome the shortcomings of clogging and loss of microspheres, which typically occurred in currently used structures for microsphere capture. A sandwich immunoassay for the detection of typical cancer biomarker CEA was successfully performed on the microfluidic device with all reagents reacted together, which led to reduced experimental time and simple operations. As a result, a limit of detection of 0.5ng/mL for carcinoma-embryonic antigen detection was achieved, and the calibration curve fitted the Langmuir model, which showed high clinical consistency with serum sample detection. To further investigate the performance of our system, trap efficiency and uniform ratio of microfluidic structure, and specificity of the experiment were evaluated and showed good consequences. The proposed microfluidic immunoassay system with advantages of high sensitivity and specificity, reduced time cost, and facile operations, indicates a promising potential in clinical diagnosis.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2017.11.150