A non-invasive procedure based on brush sampling and Bisulfite sequencing of a 13-gene panel to study high risk patients to develop oral cancer

A genetic procedure for detection of patients with a significant risk to develop an oral neoplasia is an attractive strategy to reduce the burden of OsCC. Recently our research group developed a non-invasive method to detect high-risk OSCC lesions based on oral brushing as method for collecting samp...

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Bibliographic Details
Published in:Annali di stomatologia 2017-01, Vol.8, p.39-39
Main Authors: Rossi, R, Morandi, L, Tarsitano, A, Gabusi, A, Gissi, D B
Format: Article
Language:English
Subjects:
Bisulfite
Brushing
Cancer
Chi-square test
Deoxyribonucleic acid
DNA
DNA methylation
Dysplasia
Epidemiology
Epithelial cells
Gene sequencing
Lesions
Leukokeratosis
Lichen planus
Oral cancer
Patients
Population studies
Risk
Risk groups
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Summary:A genetic procedure for detection of patients with a significant risk to develop an oral neoplasia is an attractive strategy to reduce the burden of OsCC. Recently our research group developed a non-invasive method to detect high-risk OSCC lesions based on oral brushing as method for collecting samples followed by DNA methylation analysis (1,2). The purpose of this study is to apply this non-invasive procedure in three different groups of patients: a group of healthy donors, a group of patients with potentially malignant lesions (OPML) and a group of patients treated for oral cancer. Aim of the study is to evaluate the between-group differences and the epidemiologic, clinical and histological variables that may influence the methylation profile in each group. Oral brushing samples were collected from 54 healthy donors, 41 patients with OPML (27 Oral leukoplakia "OL" and 14 Lichen Planus "LP") and 22 patients surgically treated for OSCC (ex-OSCC). In all cases DNA methylation analysis was applied as previously described (1). Each sample was considered positive or negative in relationship to a pre-definite cut off value. One way ANOVA analysis with multiple range test and Chi square analysis were usad to evaluate the presence of any between-group significant difference and the variables that may influence the methylation profile in each group. None of healthy donors was detected as positive, whereas 19/27 (70.3%) of patients with OL, 2/14 (14.3%) with LP and 7/22 (31.8%) ex-OSCC showed higher values with respect to cut off. OLs showed significant (p
ISSN:1824-0852
1971-1441