miRNA profiling expression from oral brushing in patients with potentially malignant disorders

MicroRNAs are short non-coding RNAs that regulate gene expression and are crucial to oral tumorigenesis; miRNAs possess unique properties that make them promising markers to be used in screening tests associated with non invasive collecting procedures. So far, few studies have described the profile...

Full description

Saved in:
Bibliographic Details
Published in:Annali di stomatologia 2017-01, Vol.8, p.13-14
Main Authors: Sozzi, L, Gabusi, A, Scapoli, L, Morandi, L, Gissi, D B
Format: Article
Language:English
Subjects:
Biomarkers
Brushing
Carcinogenesis
Carcinogens
Diagnosis
Disorders
Epithelial cells
Gene expression
Genetic transformation
Leukokeratosis
Lichen planus
MicroRNAs
miRNA
Oral cancer
Patients
Ribonucleic acid
RNA
Statistical analysis
Statistical tests
Tumorigenesis
Variance analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs are short non-coding RNAs that regulate gene expression and are crucial to oral tumorigenesis; miRNAs possess unique properties that make them promising markers to be used in screening tests associated with non invasive collecting procedures. So far, few studies have described the profile of miRNAs in Oral Potentially Malignant Disorders (OPMD). Aim of the present study was to analyze the expression of a panel of miRNAs in epithelial cells sampled by oral brushing from patients with Oral Leukoplakia (OL) and Lichen Planus (LP). Oral brushing specimens were collected from 30 patients with diagnosis of OL and 17 patients with LP. All cases underwent histological analysis; oral brushing specimens from 20 patients with a histological diagnosis of OSCC were used as positive controls, while oral brushing specimens from 24 healthy donors were used as negative controls. In all groups the expression levels of miRNAs were evaluated by real time PCR. Seven target were evaluated (miR-21, miR-375, miR-345, miR-181 b, miR146a, miR-649, miR-518b). The miRNA expression levels across groups were assessed by ANOVA statistics and Tahmane post-hoc test. ANOVA analysis showed a between-group significant difference as regards miR146a (F=15.048, p
ISSN:1824-0852
1971-1441