Comparative effects of various nitric oxide donors on ferritin regulation, programmed cell death, and cell redox state in plant cells

• Published in: Journal of plant physiology 2004-07, Vol.161 (7), p.777-783
• Main Authors: Murgia, Irene, Concetta de Pinto, Maria, Delledonne, Massimo, Soave, Carlo, De Gara, Laura
• Format: Article
• Language: English
• Subjects: 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene; antioxidant activity; apoptosis; Apoptosis - drug effects; Arabidopsis - cytology; Arabidopsis - drug effects; Arabidopsis - metabolism; Arabidopsis thaliana; Ascorbate peroxidase; ascorbic acid; bioaccumulation; Biological and medical sciences; Catalase; Cell physiology; Cells, Cultured; cultured cells; Ferritin; Ferritins - drug effects; Ferritins - genetics; Ferritins - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; glutathione; messenger RNA; Molecular and cellular biology; Nicotiana - cytology; Nicotiana - drug effects; Nicotiana - metabolism; Nicotiana tabacum; Nitric oxide; Nitric Oxide - biosynthesis; nitric oxide donors; Nitric Oxide Donors - pharmacology; Nitroprusside - pharmacology; nitroso compounds; Nitroso Compounds - pharmacology; NO-donor; Oxidation-Reduction - drug effects; Penicillamine - analogs & derivatives; Penicillamine - pharmacology; Plant Cells; Plants - drug effects; Plants - metabolism; Programmed cell death; Reactive Oxygen Species; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; redox reactions; S-nitroso-N-acetylpenicillamine; S-nitrosoglutathione; S-Nitrosoglutathione - pharmacology; Signal transduction; sodium nitroprusside
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2003.12.004

Past studies investigating the regulatory functions of nitric oxide (NO) in plant cells have utilized various NO-donors that release NO in different redox forms, which has lead to problems in the interpretation of data. In the present study, the effects of different NO-donors releasing NO with either NO + (SNP) or NO (SNAP, GSNO, NOC-18) character have been compared in plant cells. In particular, ferritin regulation, programmed cell death, cellular redox state, and ROS-scavenging enzymes in Arabidopsis thaliana and Nicotiana tabacum cells were examined. The results show that SNP behaves differently than the other NO-donors tested; indeed, SNP induces accumulation of ferritin transcripts in Arabidopsis, whereas SNAP inhibits its accumulation. Moreover, among the assortment of donors tested, only SNP caused programmed cell death and suppression of ROS-scavenging systems.