Selenised yeast sources differ in their capacity to protect porcine jejunal epithelial cells from cadmium-induced toxicity and oxidised DNA damage

In recent years there has been increasing interest in the use of selenised yeast (Se-Y) as an antioxidant feed supplement. Here, three selenised yeast products are differentiated in terms of bioefficiency and the ameliorative effect on Cadmium (Cd) toxicity in porcine epithelial cells. A porcine dig...

Full description

Saved in:
Bibliographic Details
Published in:Biometals 2018-10, Vol.31 (5), p.845-858
Main Authors: Lynch, Sarah, Horgan, Karina, Walls, Dermot, White, Blánaid
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Bioassays
Bioavailability
Biochemistry
Biomedical and Life Sciences
Cadmium
Cell Biology
Computer simulation
Damage assessment
Deoxyribonucleic acid
DNA
DNA damage
DNA fragmentation
DNA nucleotidylexotransferase
DNA repair
Epithelial cells
Feed supplements
Glutathione
Glutathione peroxidase
Life Sciences
Medicine/Public Health
Microbiology
Peroxidase
Pharmacology/Toxicology
Plant Physiology
Reductase
Repair
Selenium
Thioredoxin
Toxicity
Yeast
Yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In recent years there has been increasing interest in the use of selenised yeast (Se-Y) as an antioxidant feed supplement. Here, three selenised yeast products are differentiated in terms of bioefficiency and the ameliorative effect on Cadmium (Cd) toxicity in porcine epithelial cells. A porcine digestion in vitro model was chosen to more accurately simulate the bioavailability of different Se-Y preparations, allowing a comprehensive understanding of the bio efficiency of each Se-Y compound in the porcine model. To elucidate a possible mechanism of action of selenium a number of bioassays were applied. Levels of Se dependent antioxidant enzymes (glutathione peroxidase and thioredoxin reductase) were evaluated to analyze the ROS neutralizing capacity of each Se-Y compound. The effects of Se-Y sources on Cd-induced DNA damage and apoptosis-associated DNA fragmentation was assessed using comet and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays, respectively. Lesion-specific DNA damage analysis and in vitro DNA repair assay determined the DNA repair capacity of each Se-Y source. The results presented in this study confirm that the ability of different commercially available Se-Y preparations to enhance a range of cellular mechanisms that protect porcine gut epithelial cells from Cd-induced damage is concentration-dependent and illustrates the difference in bioefficiency of different Se-Y compounds.
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-018-0129-z