Molecular Cloning and Expression Analysis of Vps26A Gene from Deer Antler Tip of Different Growth Stages

Retromer complex plays a crucial role in retrograde transport of the recycle proteins from the endosome to Golgi, and Vps26A protein is an important component of the retromer complex. In the present study cDNA sequence of the full coding region of the Vps26A gene was successfully cloned from antler...

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Bibliographic Details
Published in:Pakistan journal of zoology 2018-06, Vol.50 (3), p.825
Main Authors: Xia, Yanling, Li, Heping, Liang, Yuntao, Zhao, Jichen, Lu, Binshan, Liu, Di
Format: Article
Language:English
Subjects:
Amino acid sequence
Amino acids
Antlers
Biomedical materials
Bos taurus
Cervus nippon hortulorum
Cloning
Deer
Disease
Gene expression
Genetic aspects
Glutamic acid
Golgi apparatus
Growth rate
Homology
Mammals
Nucleotide sequence
Phylogenetics
Phylogeny
Physiological aspects
Polymerase chain reaction
Proteins
Retrograde transport
Sika deer
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Summary:Retromer complex plays a crucial role in retrograde transport of the recycle proteins from the endosome to Golgi, and Vps26A protein is an important component of the retromer complex. In the present study cDNA sequence of the full coding region of the Vps26A gene was successfully cloned from antler tip of the Sika deer (Cervus nippon hortulorum). The Vps26A cDNA contains an open reading frame of 984bp encoding a polypeptide with 327 amino acids. The deduced molecular mass and isoelectric point of Vps26A protein were 38.2 kDa and 6.13, respectively. Glutamic acid had the largest proportion (10.4%) in the primary structure. Homologous sequence alignment and phylogenetic tree analysis indicated that theVps26A protein of sika deer was highly similar to that of Bos taurus. Expression analysis by real-time quantitative RT-PCR revealed that Vps26A gene had a higher expression level at day 90 than those obtained at day 60 and 30.
ISSN:0030-9923
DOI:10.17582/journal.pjz/2018.3.825.830