Inhibitory effect of Thai propolis on human osteoclastogenesis

Background/Aim Avulsed teeth should be immediately replanted into the socket or otherwise kept in a physiologic storage medium to maintain periodontal ligament cell viability. A previous study has demonstrated that Thai propolis extract can maintain viability of human periodontal ligament cells. How...

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Published in:Dental traumatology 2018-08, Vol.34 (4), p.237-244
Main Authors: Wimolsantirungsri, Nattaporn, Makeudom, Anupong, Louwakul, Phumisak, Sastraruji, Thanapat, Chailertvanitkul, Pattama, Supanchart, Chayarop, Krisanaprakornkit, Suttichai
Format: Article
Language:English
Subjects:
Acid phosphatase (tartrate-resistant)
Actin
Dentin
Hemopoiesis
Leukocytes (mononuclear)
Osteoclastogenesis
Osteoclasts
Osteoprogenitor cells
Periodontal ligament
Peripheral blood mononuclear cells
Phosphatase
Polymerase chain reaction
Propolis
Root resorption
tartrate‐resistant acid phosphatase
Teeth
tooth avulsion
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Summary:Background/Aim Avulsed teeth should be immediately replanted into the socket or otherwise kept in a physiologic storage medium to maintain periodontal ligament cell viability. A previous study has demonstrated that Thai propolis extract can maintain viability of human periodontal ligament cells. However, root resorption by osteoclasts often occurs when the avulsed teeth are replanted. The aim of this study was to determine the inhibitory effect of Thai propolis extract on human osteoclastogenesis in vitro. Materials and methods Human peripheral blood mononuclear cells were isolated for osteoclast precursors and cultured in the presence or absence of various non‐toxic concentrations of propolis extract, as determined by the alamarBlue® assay, during in vitro induction of osteoclastogenesis. Osteoclast formation was examined by tartrate‐resistant acid phosphatase staining, actin ring formation, and real‐time polymerase chain reaction. The resorption pit assay was performed to determine osteoclast function. Results Non‐toxic concentrations of propolis extract suppressed osteoclast formation by significantly decreasing the percentages of tartrate‐resistant acid phosphatase‐positive multinuclear cells and the ratios of cells with F‐actin ring formation (P 
ISSN:1600-4469
1600-9657
DOI:10.1111/edt.12401