Genotyping of Triticum ssp. Hexaploid Species Samples with ISSR-Markers

The genetic improvement of soft wheat ( Triticum aestivum L.) and other hexaploid wheat species ( T. spelta, T. spherococcum, T. petropavlovskyi , and T. compactum ) is important. This can be done by transferring genes of interest (resistance to biotic and abiotic factors). For this purpose, phyloge...

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Bibliographic Details
Published in:Cytology and genetics 2018-07, Vol.52 (4), p.276-282
Main Authors: Kyrienko, A. V., Rozhkov, R. V., Parii, M. F., Symonenko, Yu. V.
Format: Article
Language:English
Subjects:
Abiotic factors
Biomedical and Life Sciences
Biomedicine
Gene polymorphism
Genotyping
Human Genetics
Phylogenetics
Phylogeny
Triticum aestivum
Triticum compactum
Triticum petropavlovskyi
Triticum spelta
Triticum spherococcum
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Summary:The genetic improvement of soft wheat ( Triticum aestivum L.) and other hexaploid wheat species ( T. spelta, T. spherococcum, T. petropavlovskyi , and T. compactum ) is important. This can be done by transferring genes of interest (resistance to biotic and abiotic factors). For this purpose, phylogenetic connections of the studied genotypes should be investigated. This problem can be solved by using a multilocus system based on ISSR markers. The marker system is highly polymorphic and convenient for analysis. The study presents the results of a comparison of 20 different hexaploid wheat genotypes based on ISSR markers. In addition, the level of polymorphism was determined and a dendrogram reflecting the phylogenetic connections between the studied genotypes was constructed. It was shown that the species located next to each other according to the systematics turned out to be more remote after the ISSR-marker analysis, and, vice versa, the species that were systematically more distant showed a higher level of kinship.
ISSN:0095-4527
1934-9440
DOI:10.3103/S0095452718040047