Hemoglobin as a Diagnosing Molecule for Biological Effects of Atmospheric-Pressure Plasma

The studies with proteins are necessary to understand the biological effects of atmospheric pressure plasma (APP). Among proteins, those with transient metal ions play key roles in many biological events and they are very sensitive to environmental redox states. Iron-containing hemoglobin (Hb) is in...

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Bibliographic Details
Published in:Plasma chemistry and plasma processing 2018-09, Vol.38 (5), p.937-952
Main Authors: Ki, Se Hoon, Sin, Somin, Shin, Jae-Ho, Kwon, Young Wan, Chae, Myoung Won, Uhm, Han Sup, Baik, Ku Youn, Choi, Eun Ha
Format: Article
Language:English
Subjects:
Absorption spectra
Biological effects
Characterization and Evaluation of Materials
Chemical reactions
Chemistry
Chemistry and Materials Science
Classical Mechanics
Hemoglobin
Inorganic Chemistry
Mechanical Engineering
Molecular chains
Organic chemistry
Original Paper
Pressure effects
Proteins
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Summary:The studies with proteins are necessary to understand the biological effects of atmospheric pressure plasma (APP). Among proteins, those with transient metal ions play key roles in many biological events and they are very sensitive to environmental redox states. Iron-containing hemoglobin (Hb) is investigated in this study, after APP treatments under two environmental gas conditions of pure N 2 and N 2  + O 2 mixture. Monitoring the intensity change for absorption spectra could lead to a quantitative assessment of the effect of discharge plasma on Hb. Redox states of Hb are classified into five states including O 2 -bound Hb (oxy-Hb), deoxy-Hb, met-Hb, NO-bound Hb (NO-Hb), and hemichrome. Chemically generated reactive species and some scavengers are applied to understand the chemical reactions. Our experimental results confirm the complex chemical reactions of APP and suggest the possible use of Hb as a model protein for the visualization of APP biological effects.
ISSN:0272-4324
1572-8986
DOI:10.1007/s11090-018-9917-z