Periplasmically located [alpha]-santonin binding factor in Sphingomonas paucimobilis strain S ATCC 43388

A marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking Sphingomonas paucimobilis strain S ATCC 43388 cells by freeze -- thaw method. The shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the sub...

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Bibliographic Details
Published in:Indian journal of microbiology 2007-12, Vol.47 (4), p.358
Main Authors: Raj, M Shailaja, Furtado, I, Mavinkurve, S
Format: Article
Language:English
Subjects:
Microbiology
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Summary:A marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking Sphingomonas paucimobilis strain S ATCC 43388 cells by freeze -- thaw method. The shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the substrate. Addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin to near normal.[PUBLICATION ABSTRACT]
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-007-0064-0