Vignetting effect in Fourier ptychographic microscopy

• Published in: arXiv.org 2017-10
• Main Authors: Pan, An, Zuo, Chao, Xie, Yuege, Zhang, Yan, Lei, Ming, Yao, Baoli
• Format: Article
• Language: English
• Subjects: Field of view; Image reconstruction; Invariants; Microscopy; Transfer functions; Vignetting
• ISSN: 2331-8422
• DOI: 10.48550/arxiv.1710.06717

Fourier ptychographic microscopy (FPM) is a computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular diversity illuminations. In the usual model of FPM, the microscopic system is approximated by being taken as space-invariant with transfer function determined by a complex pupil function of the objective. However, in real experimental conditions, several unexpected "semi-bright and semi-dark" images with strong vignetting effect can be easily observed when the sample is illuminated by the LED within the "transition zone" between bright field and dark field. These imperfect images, apparently, are not coincident with the space-invariant model and could deteriorate the reconstruction quality severely. In this Letter, we examine the impact of this space-invariant approximation on FPM image formation based on ray-based and rigorous wave optics-based analysis. Our analysis shows that for a practical FPM microscope with a low power objective and a large field of view, the space invariance is destroyed by diffraction at other stops associated with different lens elements to a large extent. A modified version of the space-variant model is derived and discussed. Two simple countermeasures are also presented and experimentally verified to bypass or partially alleviate the vignetting-induced reconstruction artifacts.