Association of MTNR1A and CYP19 genes polymorphisms with sperm quality and testicular size in Sanjabi breed rams

• Published in: Annals of animal science 2018-07, Vol.18 (3), p.699-711
• Main Authors: Kianpoor, Somayeh, Abdolmohammadi, Alireza, Hajarian, Hadi, Nikousefat, Zahra, Khamisabadi, Hasan
• Format: Article
• Language: English
• Subjects: Aromatase; Blood; CYP19 gene; Cytochrome; Cytochrome P450; Cytochromes P450; Deoxyribonucleic acid; Digestion; DNA; Endonuclease; Fertility; Fragmentation; Genes; Genotypes; Loci; Melatonin; MTNR1A gene; Mutation; Polymerase chain reaction; Primers; Rams; Restriction fragment length polymorphism; Sanjabi sheep; Sperm; sperm quality; Statistical tests; Testes; testicular size
• ISSN: 2300-8733; 1642-3402; 2300-8733
• DOI: 10.2478/aoas-2018-0021

The purpose of this study was to identify mutations in melatonin receptor 1A (MTNR1A) and aromatase cytochrome P450 (CYP19) genes using PCR-RFLP technique and their associations with sperm quality and testicular size traits in Sanjabi breed rams. The blood and sperm samples were collected from Sanjabi rams (n = 96). Genomic DNA was extracted from the blood. A 824bp fragment from exon II of MTNR1A gene and a 517bp fragment from promoter 2 (P2) of CYP19 gene were amplified using two pairs of specific primers. The PCR products were separately digested by two restriction enzymes, SsiI for MTNR1A locus and DraI for CYP19 locus. Digestion by SsiI restriction enzyme resulted in CC, CA and AA genotypes with frequency of 0.45, 0.41 and 0.14, respectively. However, digestion of 517bp fragment of CYP19 gene by DraI endonuclease determined two AG and AA genotypes with frequency 0.89 and 0.11, respectively. The Chi-square test proved that the two loci were in Hardy-Weinberg equilibrium (HWE). The significant effect was observed between different genotypes of MTNR1A gene and morphological trait (P