Colorimetric and NIR fluorescence receptors for F^sup -^ ion detection in aqueous condition and its Live cell imaging

• Published in: Sensors and actuators. B, Chemical Chemical, 2018-02, Vol.255, p.3194
• Main Authors: Vinoth Kumar, Gujuluva Gangatharan, Kesavan, Mookkandi Palsamy, Sivaraman, Gandhi, Rajesh, Jegathalaprathaban
• Format: Article
• Language: English
• Subjects: Biosensors; Chemical synthesis; Colorimetry; Density functional theory; Fluorescence; Fluorides; NMR; Nuclear magnetic resonance; Quantum theory; Receptors; Silicon dioxide; Single crystals; Titration; X-ray diffraction
• ISSN: 0925-4005; 1873-3077

In this paper, a new kind of colorimetric receptors R1 and R2 for fluoride ions have been synthesized and characterized by various spectral techniques. Single crystal X-ray diffraction study confirms, the receptor R2 possesses the orthorhombic centrosymmetric lattice with the donor atoms of ONS. In naked eye, absorption and fluorescence studies were evidently predicting the potential sensing capability of the receptors R1 and R2 that detect fluoride ions by competing with various ions. The detection limit was found to be more or less same as low as 10−7 M for R1 and R2 with F−. We proposed the 1:1 binding formation of R1 and R2 by means of F− ion was further supported by NMR titration and Job’s plot analysis. Besides, the density functional theory (DFT) calculations have been theoretically sustained with the sensing mechanism. Moreover, both the two receptors had the possibility of potentially applied in an analytical application for detecting the trace amounts of fluoride ion in the real water sample analysis was demonstrated. Live cell imaging experiments with the receptors displayed that these receptors extensively applicable to detect fluoride ions in HeLa cells under physiological conditions. Finally, the use of test strips containing R1 and R2 that sense fluoride ions in solution medium and supported on solid medium (silica) was exhibited.