Ordered Assembly of Mitochondria During Rice Germination Begins with Promitochondrial Structures Rich in Components of the Protein Import Apparatus

Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound promitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundanc...

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Bibliographic Details
Published in:Plant molecular biology 2006, Vol.60 (2), p.201-223
Main Authors: Howell, K.A, Millar, A.H, Whelan, J
Format: Article
Language:English
Subjects:
cell biology
cell membranes
cell respiration
Cristae
Developmental stages
Electron transport chain
embryo (plant)
Embryos
gene expression
Genomes
Germination
imbibition
Maturation
messenger RNA
Mitochondria
mitochondrial proteins
NADH
Organic acids
Oryza sativa
Oxidation
Plant cells
plant proteins
promitochondria
promitochondrial structures
protein import apparatus
Protein transport
Proteins
rice
seed germination
Seeds
Transcription
Tricarboxylic acid cycle
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Summary:Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound promitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts encoding mitochondrial proteins was observed. Co-incident with these changes in transcript levels was dynamic and rapid changes in mitochondrial protein content and mitochondrial function. Proteins representing components of the mitochondrial protein import apparatus are strikingly abundant in dry seeds, and a functional import apparatus was shown to operate 2 h after imbibition. Interestingly, this import process was best driven by the oxidation of NADH from outside the mitochondrial inner membrane. In later developmental stages the capacity for matrix organic acid metabolism was evident, accompanied by the appearance of proteins for TCA cycle components, and coordination of electron transport chain assembly through components encoded in both mitochondrial and nuclear genomes. Together these events provide new insights into the understanding of mitochondrial maturation and the nature of promitochondrial structures in plant cells.
ISSN:0167-4412
1573-5028
DOI:10.1007/s11103-005-3688-7