Development of an Optical Urea Biosensor Using Polypyrrole-polyvinyl Sulphonate Film

Development of an intensity modulated fiber optic PPy-PVS biosensor for the detection of urea has been presented. The sensor design is based on the modified cladding technique. Polypyrrole (PPy) film doped with polyvinyl sulfonate (PVS) was synthesized (by in-situ chemical polymerization at room tem...

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Bibliographic Details
Published in:Sensors & transducers 2009-02, Vol.101 (2), p.112
Main Authors: Kharat, H J, Datta, K, Ghosh, P, Shirsat, Mahendra D
Format: Article
Language:English
Subjects:
Biosensors
Design
Fiber optics
Polymer films
Research methodology
Scanning electron microscopy
Urine
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Summary:Development of an intensity modulated fiber optic PPy-PVS biosensor for the detection of urea has been presented. The sensor design is based on the modified cladding technique. Polypyrrole (PPy) film doped with polyvinyl sulfonate (PVS) was synthesized (by in-situ chemical polymerization at room temperature) with optimized process parameters. The PPy-PVS film provides good porous matrix for the immobilization of enzyme-urease on the sensing probe by cross linking via glutaraldehyde. The characterization of the urea biosensor has been carried out with an indigenously developed sensing chamber and optical fiber test bench. This biosensor showed almost stable and linear response to urea in the range 1-100 mM up to 24 days. It shows very good selectivity and repeatability. [PUBLICATION ABSTRACT]
ISSN:2306-8515
1726-5479