IL-1/inhibitory κB kinase ε–induced glycolysis augment epithelial effector function and promote allergic airways disease

• Published in: Journal of allergy and clinical immunology 2018-08, Vol.142 (2), p.435-450.e10
• Main Authors: Qian, Xi, Aboushousha, Reem, van de Wetering, Cheryl, Chia, Shi B., Amiel, Eyal, Schneider, Robert W., van der Velden, Jos L.J., Lahue, Karolyn G., Hoagland, Daisy A., Casey, Dylan T., Daphtary, Nirav, Ather, Jennifer L., Randall, Matthew J., Aliyeva, Minara, Black, Kendall E., Chapman, David G., Lundblad, Lennart K.A., McMillan, David H., Dixon, Anne E., Anathy, Vikas, Irvin, Charles G., Poynter, Matthew E., Wouters, Emiel F.M., Vacek, Pamela M., Henket, Monique, Schleich, Florence, Louis, Renaud, van der Vliet, Albert, Janssen-Heininger, Yvonne M.W.
• Format: Article
• Language: English
• Subjects: Age; Animal models; Animals; Antigens, Dermatophagoides - immunology; Asthma; Asthma - metabolism; Body mass index; Cells, Cultured; Cohort Studies; Dehydrogenase; Dehydrogenases; Disease Models, Animal; Enzyme inhibitors; Epithelial cells; Epithelium; Female; Glycolysis; Granulocyte-macrophage colony-stimulating factor; Health care; House dust; house dust mite; Humans; Hypersensitivity - metabolism; I-kappa B Proteins - metabolism; IL-1; Inflammation; inhibitor of κB kinase ε; Interleukin 1; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; L-Lactate dehydrogenase; Laboratories; lactate; lactate dehydrogenase A; Lactic acid; Lactic Acid - metabolism; Leukocytes (neutrophilic); Lung - metabolism; Lung - pathology; Lungs; Male; Metabolism; Mice; Middle Aged; Neutrophils - pathology; Nose - pathology; Pathogenesis; Phosphatase; Proto-Oncogene Proteins - metabolism; Pyroglyphidae; Respiratory function; Respiratory Mucosa - metabolism; Respiratory Mucosa - pathology; Respiratory tract; RNA, Small Interfering - genetics; Signal Transduction; siRNA; Software; Sputum; Sputum - metabolism; Thymic stromal lymphopoietin; Thymus
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2017.08.043

Emerging studies suggest that enhanced glycolysis accompanies inflammatory responses. Virtually nothing is known about the relevance of glycolysis in patients with allergic asthma. We sought to determine whether glycolysis is altered in patients with allergic asthma and to address its importance in the pathogenesis of allergic asthma. We examined alterations in glycolysis in sputum samples from asthmatic patients and primary human nasal cells and used murine models of allergic asthma, as well as primary mouse tracheal epithelial cells, to evaluate the relevance of glycolysis. In a murine model of allergic asthma, glycolysis was induced in the lungs in an IL-1–dependent manner. Furthermore, administration of IL-1β into the airways stimulated lactate production and expression of glycolytic enzymes, with notable expression of lactate dehydrogenase A occurring in the airway epithelium. Indeed, exposure of mouse tracheal epithelial cells to IL-1β or IL-1α resulted in increased glycolytic flux, glucose use, expression of glycolysis genes, and lactate production. Enhanced glycolysis was required for IL-1β– or IL-1α–mediated proinflammatory responses and the stimulatory effects of IL-1β on house dust mite (HDM)–induced release of thymic stromal lymphopoietin and GM-CSF from tracheal epithelial cells. Inhibitor of κB kinase ε was downstream of HDM or IL-1β and required for HDM-induced glycolysis and pathogenesis of allergic airways disease. Small interfering RNA ablation of lactate dehydrogenase A attenuated HDM-induced increases in lactate levels and attenuated HDM-induced disease. Primary nasal epithelial cells from asthmatic patients intrinsically produced more lactate compared with cells from healthy subjects. Lactate content was significantly higher in sputum supernatants from asthmatic patients, notably those with greater than 61% neutrophils. A positive correlation was observed between sputum lactate and IL-1β levels, and lactate content correlated negatively with lung function. Collectively, these findings demonstrate that IL-1β/inhibitory κB kinase ε signaling plays an important role in HDM-induced glycolysis and pathogenesis of allergic airways disease. [Display omitted]