Immuno-PCR Assay for Quantitation of Antibodies to Epstein–Barr Virus

Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein–Barr virus (EBV) in...

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Bibliographic Details
Published in:Molecular biology (New York) 2018-07, Vol.52 (4), p.629-635
Main Authors: Pivovarov, V. D., Ryazantsev, D. Yu, Simonova, M. A., Yegorova, T. V., Khlgatian, S. V., Zavriev, S. K., Svirshchevskaya, E. V.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotin
Enzyme-linked immunosorbent assay
Epstein-Barr virus
Human Genetics
Immunoglobulin G
Immunoglobulins
Life Sciences
Methods
Quantitation
Sensitivity
Streptavidin
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Summary:Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein–Barr virus (EBV) in the human blood serum. EBV nuclear protein 1 fragment (pEBV) was expressed in Escherichia coli . A synthetic single-stranded deoxyribonucleotide was conjugated to streptavidin, and the conjugate was used to detect рEBV–IgG1–biotin complexes by qiPCR. The IgG1 titers determined by qiPCR were compared to the results of enzyme-linked immunosorbent assay (ELISA). The sensitivity of qiPCR was one order of magnitude higher than that of ELISA. Thus, a highly sensitive qiPCR-based assay was developed to quantitate antibodies specific to the recombinant EBV antigen.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893318040155