Synthesis of 3-[3- phenylpyrazylazo ]-2, 7- naphthalendiol as new chromogenic reagent for the determination of Nickel (II) in Nails of Human

• Published in: Research journal of pharmacy and technology 2018-04, Vol.11 (4), p.1566-1572
• Main Authors: Abdulhameed, Abdulhameed M., Abedalkadeem, Mustafa, Mohammed, Hussain J.
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Bioassays; Biological activity; Ethanol; Ligands; Nickel
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.5958/0974-360X.2018.00292.5

The procedure was found to be convenient for the routine analysis of trace amount of Nickel (II) in aqueous media. The method is based on the formation of a 1:2 complex with 3-(3-phenylpyrazylazo)2,7-naphthalendiol (3PPAN) as a new reagent is advanced. The complex has a maximum absorption at 501 nm and ε max of 4x104 L. mol-1. cm-1 A linear correlation (2.5 -20. 0 µg. ml-1) was found between absorbance at 1 max and concentration. The accuracy and reproducibility of the determination method for various known amounts of Nickel (II) were tested. The results obtained are both precise (RSD was 2.50%) and accurate (relative error was 2.50 %). The effect of diverse ions on the determination of Nickel (II) to investigate the selectivity of the method were also studied. The stability constant of the product was 0.41X1010 L. mol-1. The method was successfully utilized to the limitation of Ni (II) ion in analytical and nails samples