High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

[Display omitted] •Marasmiellus palmivorus VE111 is an excellent laccase secretor.•Carbon and nitrogen sources influence the production of ligninolytic enzymes.•Glucose and casein were shown to be the best sources for ligninolytic enzymes.•Lower glucose concentrations provided higher laccase activit...

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Bibliographic Details
Published in:Process biochemistry (1991) 2018-06, Vol.69, p.1-11
Main Authors: Schneider, Willian Daniel Hahn, Fontana, Roselei Claudete, Mendonça, Simone, de Siqueira, Félix Gonçalves, Dillon, Aldo José Pinheiro, Camassola, Marli
Format: Article
Language:English
Subjects:
Bioreactor
Bioreactors
Biorefinery
Carbon
Carbon sources
Casein
Enzymes
Experimental design
Fungi
Glucose
Ligninolytic enzymes
Lignocellulose
Manganese
Manganese peroxidase
Nitrogen
Nitrogen sources
Peroxidase
Rot
Submerged cultivation
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Summary:[Display omitted] •Marasmiellus palmivorus VE111 is an excellent laccase secretor.•Carbon and nitrogen sources influence the production of ligninolytic enzymes.•Glucose and casein were shown to be the best sources for ligninolytic enzymes.•Lower glucose concentrations provided higher laccase activity.•Laccase production showed enzymatic kinetics associated with growth. The present work was carried out to determine the best sources and concentrations of carbon and nitrogen in the production of laccases, total peroxidases and manganese peroxidase by Marasmiellus palmivorus VE111. Among the carbon and nitrogen sources used, the combination of glucose and casein led to higher production of laccases (5134 U/mL), total peroxidases (187 U/mL) and manganese peroxidase (57 U/mL). Central composite rotatable design (CCRD) was used to determine the optimum concentrations of glucose and casein to produce enzymes. The experiments with better results were scaled up in a 5 L bioreactor. It was verified that lower concentrations of glucose (5 and 10 g/L) stimulated higher activity of total peroxidases (1285 U/mL) and laccases (3420 U/mL), respectively, while a higher concentration of glucose (30 g/L) favoured the production of manganese peroxidase (59 U/mL). These data indicate high performance of M. palmivorus VE111 in the production of ligninolytic enzymes.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2018.03.005