High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

• Published in: Process biochemistry (1991) 2018-06, Vol.69, p.1-11
• Main Authors: Schneider, Willian Daniel Hahn, Fontana, Roselei Claudete, Mendonça, Simone, de Siqueira, Félix Gonçalves, Dillon, Aldo José Pinheiro, Camassola, Marli
• Format: Article
• Language: English
• Subjects: Bioreactor; Bioreactors; Biorefinery; Carbon; Carbon sources; Casein; Enzymes; Experimental design; Fungi; Glucose; Ligninolytic enzymes; Lignocellulose; Manganese; Manganese peroxidase; Nitrogen; Nitrogen sources; Peroxidase; Rot; Submerged cultivation
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2018.03.005

[Display omitted] •Marasmiellus palmivorus VE111 is an excellent laccase secretor.•Carbon and nitrogen sources influence the production of ligninolytic enzymes.•Glucose and casein were shown to be the best sources for ligninolytic enzymes.•Lower glucose concentrations provided higher laccase activity.•Laccase production showed enzymatic kinetics associated with growth. The present work was carried out to determine the best sources and concentrations of carbon and nitrogen in the production of laccases, total peroxidases and manganese peroxidase by Marasmiellus palmivorus VE111. Among the carbon and nitrogen sources used, the combination of glucose and casein led to higher production of laccases (5134 U/mL), total peroxidases (187 U/mL) and manganese peroxidase (57 U/mL). Central composite rotatable design (CCRD) was used to determine the optimum concentrations of glucose and casein to produce enzymes. The experiments with better results were scaled up in a 5 L bioreactor. It was verified that lower concentrations of glucose (5 and 10 g/L) stimulated higher activity of total peroxidases (1285 U/mL) and laccases (3420 U/mL), respectively, while a higher concentration of glucose (30 g/L) favoured the production of manganese peroxidase (59 U/mL). These data indicate high performance of M. palmivorus VE111 in the production of ligninolytic enzymes.